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Literature summary for 2.3.3.16 extracted from

  • Murphy, J.R.; Donini, S.; Kappock, T.J.
    An active site-tail interaction in the structure of hexahistidine-tagged Thermoplasma acidophilum citrate synthase (2015), Acta Crystallogr. Sect. F, 71, 1292-1299.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M CHES pH 9.5, 12% (w/v) PEG 4000, 1 mM oxaloacetate Thermoplasma acidophilum

Protein Variants

Protein Variants Comment Organism
H222Q the mutant shows less than 0.05% of wild type activity Thermoplasma acidophilum

Inhibitors

Inhibitors Comment Organism Structure
ACMX competitive inhibitor versus acetyl-CoA Thermoplasma acidophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 acetyl-CoA pH and temperature not specified in the publication Thermoplasma acidophilum
0.0052
-
 oxaloacetate pH and temperature not specified in the publication Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate Thermoplasma acidophilum
-
 citrate + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Thermoplasma acidophilum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-loaded iminodiacetic acid Sepharose column chromatography and Sephadex 200 gel filtration Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
 Thermoplasma acidophilum citrate + CoA
-
 ?