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Literature summary for 2.3.3.16 extracted from
- Identification and characterization of a novel citrate synthase from Streptomyces diastaticus No. 7 strain M1033 (2015), Biotechnol. Appl. Biochem., 62, 300-308.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | 5 mM AMP increases the enzyme activity to about 23% | Streptomyces diastaticus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta (DE3) cells | Streptomyces diastaticus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | 14% inhibition at 5 mM | Streptomyces diastaticus | |
| Ag+ | almost complete inhibition at 2 mM | Streptomyces diastaticus | |
| ATP | 32% inhibition at 5 mM | Streptomyces diastaticus | |
| Co2+ | 22% inhibition at 2 mM | Streptomyces diastaticus | |
| Cu2+ | 10% inhibition at 2 mM | Streptomyces diastaticus | |
| Hg2+ | 95% inhibition at 2 mM | Streptomyces diastaticus | |
| Mn2+ | 40% inhibition at 2 mM | Streptomyces diastaticus | |
| additional information | not influenced by NADH | Streptomyces diastaticus | |
| Ni2+ | 21% inhibition at 2 mM | Streptomyces diastaticus | |
| Pb2+ | almost complete inhibition at 2 mM | Streptomyces diastaticus | |
| Zn2+ | 55% inhibition at 2 mM | Streptomyces diastaticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0112 | - |
oxaloacetate | at pH 8.0 and 35°C | Streptomyces diastaticus | |
| 0.0584 | - |
acetyl-CoA | at pH 8.0 and 35°C | Streptomyces diastaticus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 116.6% activity at 2 mM | Streptomyces diastaticus | |
| additional information | the enzyme does not require monovalent or divalent metal ions for catalysis | Streptomyces diastaticus | |
| Na+ | 112.6% activity at 2 mM | Streptomyces diastaticus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, SDS-PAGE | Streptomyces diastaticus |
| 49868 | - |
2 * 49868, MALDI-TOF mass spectrometry | Streptomyces diastaticus |
| 99990 | - |
gel filtration | Streptomyces diastaticus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | Streptomyces diastaticus | - |
citrate + CoA | - |
? | |
| acetyl-CoA + H2O + oxaloacetate | Streptomyces diastaticus SM33 | - |
citrate + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces diastaticus | G8XQY5 | - |
- |
| Streptomyces diastaticus SM33 | G8XQY5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon metal (Co2+) affinity resin column chromatography | Streptomyces diastaticus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | - |
Streptomyces diastaticus | citrate + CoA | - |
? | |
| acetyl-CoA + H2O + oxaloacetate | - |
Streptomyces diastaticus SM33 | citrate + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 48000, SDS-PAGE | Streptomyces diastaticus |
| homodimer | 2 * 49868, MALDI-TOF mass spectrometry | Streptomyces diastaticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Streptomyces diastaticus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 55 | the enzyme maintains almost all of its initial activity after incubation for 20 min at 40°C or below. The half-life of the enzyme is 10 min at 50°C and is increased to 210 min in the presence of oxaloacetate. Enzyme activity is undetectable after incubation for 10 min at 55°C | Streptomyces diastaticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 230.7 | - |
oxaloacetate | at pH 8.0 and 35°C | Streptomyces diastaticus | |
| 262.8 | - |
acetyl-CoA | at pH 8.0 and 35°C | Streptomyces diastaticus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Streptomyces diastaticus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4500 | - |
acetyl-CoA | at pH 8.0 and 35°C | Streptomyces diastaticus | |
| 20700 | - |
oxaloacetate | at pH 8.0 and 35°C | Streptomyces diastaticus | |
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