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Literature summary for 2.3.3.16 extracted from

  • Zinsser, V.L.; Moore, C.M.; Hoey, E.M.; Trudgett, A.; Timson, D.J.
    Citrate synthase from the liver fluke Fasciola hepatica (2013), Parasitol. Res., 112, 2413-2417.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Hms174(DE3) cells Fasciola hepatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.22
-
oxaloacetate at pH 8.1 and 28°C Fasciola hepatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
2 * 52000, SDS-PAGE Fasciola hepatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate Fasciola hepatica
-
citrate + CoA
-
?

Organism

Organism UniProt Comment Textmining
Fasciola hepatica M4QLE7
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-affinity column chromatography Fasciola hepatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate
-
Fasciola hepatica citrate + CoA
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 52000, SDS-PAGE Fasciola hepatica

pI Value

Organism Comment pI Value Maximum pI Value
Fasciola hepatica calculated from amino acid sequence
-
8.1