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Literature summary for 2.3.3.3 extracted from

  • Gottschalk, G.
    Partial purification and some properties of the (R)-citrate synthase from Clostridium acidi-urici (1969), Eur. J. Biochem., 7, 301-106.
    View publication on PubMed
Search for more literature for 2.3.3.3

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10-20 mM, reducing reagent is required for maximal activity Clostridium acidi-urici
glutathione reducing reagent is required for maximal activity Clostridium acidi-urici
L-cysteine reducing reagent is required for maximal activity Clostridium acidi-urici

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.1 mM, required for maximal activity Clostridium acidi-urici

Organism

Organism UniProt Comment Textmining
Clostridium acidi-urici
-
-
-

Oxidation Stability

Oxidation Stability Organism
reducing reagents and anaerobic conditions are required for maximal activity Clostridium acidi-urici

Purification (Commentary)

Purification (Comment) Organism
isoelectric precipitation, DEAE-cellulose, DEAE-Sephadex Clostridium acidi-urici

Storage Stability

Storage Stability Organism
refrigerator, 24 h, 10% loss of activity Clostridium acidi-urici

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + H2O + oxaloacetate stereospecific for (R)-citrates Clostridium acidi-urici citrate + CoA
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5 sharp decrease in activity above Clostridium acidi-urici

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 approx. 50% activity at pH 7.0 Clostridium acidi-urici