Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | purified enzyme does not require 2-mecaptoethanol or anaerobic conditiones for maximal activity | Clostridium acidi-urici |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 5 mM, 64% inhibition, competitive vs. oxaloacetate | Clostridium acidi-urici | |
4-chloromercuribenzoate | strong inhibition | Clostridium acidi-urici | |
Cd2+ | complete inactivation | Clostridium acidi-urici | |
H2O2 | - |
Clostridium acidi-urici | |
Hg2+ | complete inactivation | Clostridium acidi-urici | |
iodoacetamide | strong inhibition | Clostridium acidi-urici | |
additional information | not inhibited by 5,5'-dithiobis(2-nitrobenzoate) | Clostridium acidi-urici | |
Zn2+ | above 0.02 mM, activation below | Clostridium acidi-urici |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
oxaloacetate | - |
Clostridium acidi-urici | |
0.05 | - |
acetyl-CoA | - |
Clostridium acidi-urici |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mn2+ | Clostridium acidi-urici | |
Zn2+ | activation below 0.02 mM, inhibition above | Clostridium acidi-urici |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium acidi-urici | - |
- |
- |
Purification (Comment) | Organism |
---|---|
isoelectric precipitate, DEAE-cellulose, ammonium sulfate, DEAE-Sephadex, ammonium sulfate, gel filtration | Clostridium acidi-urici |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Clostridium acidi-urici |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + H2O + oxaloacetate | no activity with propionyl-CoA, glyoxylate, pyruvate, 2-oxobutyrate, 2-oxoglutarate, 2-oxoisovalerate and 2-oxoisocaproate | Clostridium acidi-urici | citrate + CoA | - |
? |