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Literature summary for 2.3.3.3 extracted from
- Re-citrate synthase from Clostridium kluyveri is phylogenetically related to homocitrate synthase and isopropylmalate synthase rather than to Si-citrate synthase (2007), J. Bacteriol., 189, 4299-4304.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clostridium kluyveri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aerobic conditions | 50% inactivation in 24 h by aerobic conditions | Clostridium kluyveri | |
| EDTA | 0.05 mM, complete inhibition, reactivation by addition of Mg2+, Mn2+, or Co2+ | Clostridium kluyveri |  |
| p-chloromercuribenzoate | 0.002 mM, inactivation | Clostridium kluyveri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
oxaloacetate | pH 8.0 | Clostridium kluyveri | |
| 0.05 | - |
acetyl-CoA | pH 8.0 | Clostridium kluyveri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 52100, calculated, x * 51000, SDS-PAGE | Clostridium kluyveri |
| 52100 | - |
x * 52100, calculated, x * 51000, SDS-PAGE | Clostridium kluyveri |
| 52100 | - |
x * 52100, calculated from sequence | Clostridium kluyveri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium kluyveri | - |
- |
- |
| Clostridium kluyveri | - |
isoform IPMS2 | - |
| Desulfovibrio desulfuricans | - |
- |
- |
| Desulfovibrio vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Clostridium kluyveri |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| enzyme inhibited by EDTA can be reactivated by Mg2+, Mn2+, Co2+ | Clostridium kluyveri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
pH 8.0 | Clostridium kluyveri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + H2O + oxaloacetate | - |
Clostridium kluyveri | citrate + CoA | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 52100, calculated, x * 51000, SDS-PAGE | Clostridium kluyveri |
| ? | x * 52100, calculated from sequence | Clostridium kluyveri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Re-citrate synthase | - |
Clostridium kluyveri |
| Re-citrate synthase | - |
Desulfovibrio desulfuricans |
| Re-citrate synthase | - |
Desulfovibrio vulgaris |
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Release 2023.1 (January 2023)
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