General Stability | Organism |
---|---|
ATP and citrate stabilize | Lipomyces starkeyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
lauroyl-CoA | 10 mM, 52% inhibition | Lipomyces starkeyi | |
myristoyl-CoA | 10 mM, 5% inhibition | Lipomyces starkeyi | |
oleoyl-CoA | 10 mM, 21% inhibition | Lipomyces starkeyi | |
palmitoyl-CoA | 10 mM, 5% inhibition | Lipomyces starkeyi | |
stearoyl-CoA | 10 mM, 45% inhibition | Lipomyces starkeyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
citrate | - |
Lipomyces starkeyi | |
0.135 | - |
ATP | - |
Lipomyces starkeyi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 37.1% of the activation with Mg2+ at 10 mM | Lipomyces starkeyi | |
Mg2+ | required | Lipomyces starkeyi | |
Mn2+ | 22.6% of the activation with Mg2+ at 10 mM | Lipomyces starkeyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
510000 | - |
gel filtration | Lipomyces starkeyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lipomyces starkeyi | - |
CBS 1809 | - |
Lipomyces starkeyi CBS 1809 | - |
CBS 1809 | - |
Purification (Comment) | Organism |
---|---|
- |
Lipomyces starkeyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.922 | - |
- |
Lipomyces starkeyi |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl, pH 7.5, 1 mM MgCl2, 10 mM 2-mercaptoethanol, 83% loss of activity after 48 h. 52% loss of activity in presence of 1 mM ATP. 30% loss of activity in presence of 20 mM citrate, 9% loss of activity in presence of 1 mM ATP and 20 mM citrate | Lipomyces starkeyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + CoA | specific for ATP | Lipomyces starkeyi | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? | |
ATP + citrate + CoA | specific for ATP | Lipomyces starkeyi CBS 1809 | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.6 | - |
Lipomyces starkeyi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 9.5 | about 50% of maximal activity at pH 7.3 and at pH 9.5 | Lipomyces starkeyi |