Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Chlorobium limicola |
Protein Variants | Comment | Organism |
---|---|---|
H274A | mutation abolishes both the catalytic activity and phosphorylation of the enzyme by ATP | Chlorobium limicola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive inhibitor of ATP | Chlorobium limicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | a strong negative cooperativity is observed with respect to citrate binding | Chlorobium limicola | |
0.21 | - |
ATP | - |
Chlorobium limicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + CoA | Chlorobium limicola | enzyme regulates both the direction and carbon flux of the carbon dioxide-fixing reductive tricarboxylic acid cycle | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobium limicola | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Chlorobium limicola |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA | ordered mechanism where the phosphate group of ATP is first covalently bound to the His273 catalytic residue, and subsequently transferred to the citrate molecule to form citryl phosphate | Chlorobium limicola |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + citrate + CoA | the first step of the reaction is reversible | Chlorobium limicola | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
ir | |
ATP + citrate + CoA | enzyme regulates both the direction and carbon flux of the carbon dioxide-fixing reductive tricarboxylic acid cycle | Chlorobium limicola | ADP + phosphate + acetyl-CoA + oxaloacetate | - |
ir | |
dATP + citrate + CoA | 40% of the activity with ATP | Chlorobium limicola | dADP + phosphate + acetyl-CoA + oxaloacetate | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
ADP | - |
Chlorobium limicola |