Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Corynebacterium glutamicum | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Corynebacterium glutamicum | |
glycolate | - |
Corynebacterium glutamicum | |
additional information | acetyl-phosphate; not: fructose 1,6-bisphosphate | Corynebacterium glutamicum | |
oxalate | - |
Corynebacterium glutamicum | |
phosphoenolpyruvate | not | Corynebacterium glutamicum | |
pyruvate | not | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
acetyl-CoA | - |
Corynebacterium glutamicum | |
0.03 | - |
glyoxylate | - |
Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 25% of the activation with Mg2+ | Corynebacterium glutamicum | |
Mg2+ | absolute requirement for divalent cation, maximal activity with 40 mM Mg2+ | Corynebacterium glutamicum | |
Mn2+ | 15% of the activation with Mg2+ | Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Corynebacterium glutamicum |
90000 | - |
1 * 90000, SDS-PAGE | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | P42450 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.6 | - |
- |
Corynebacterium glutamicum |
Storage Stability | Organism |
---|---|
4°C, stable for more than 3 weeks | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + acetyl-CoA + H2O | - |
Corynebacterium glutamicum | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 90000, SDS-PAGE | Corynebacterium glutamicum |
monomer | 1* 82362, calculation from nucleotide sequence | Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
43 | - |
- |
Corynebacterium glutamicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
oxalate | - |
Corynebacterium glutamicum | |
0.44 | - |
glycolate | - |
Corynebacterium glutamicum | |
4.3 | - |
ATP | - |
Corynebacterium glutamicum |