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Literature summary for 2.3.3.9 extracted from

  • Loke, P.; Goh, L.L.; Soh, B.S.; Yeow, P.; Sim, T.S.
    Purification and characterization of recombinant malate synthase enzymes from Streptomyces coelicolor A3(2) and S. clavuligerus NRRL3585 (2002), J. Ind. Microbiol. Biotechnol., 28, 239-243.
    View publication on PubMed
Search for more literature for 2.3.3.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces coelicolor
expression in Escherichia coli Streptomyces clavuligerus

Inhibitors

Inhibitors Comment Organism Structure
acetyl-CoA slight reduction of enzyme activity at high concentrations of acety-CoA Streptomyces clavuligerus
acetyl-CoA slight reduction of enzyme activity at high concentrations of acety-CoA Streptomyces coelicolor
glyoxylate slight reduction of enzyme activity at high concentrations of glyoxylate Streptomyces clavuligerus
glyoxylate slight reduction of enzyme activity at high concentrations of glyoxylate Streptomyces coelicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Streptomyces coelicolor
additional information
-
 additional information
-
 Streptomyces clavuligerus
0.00059
-
 glyoxylate
-
 Streptomyces clavuligerus
0.00349
-
 glyoxylate
-
 Streptomyces coelicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Streptomyces coelicolor
Mg2+ required Streptomyces clavuligerus
Mg2+ optimal concentration 0.01 mM MgCl2 Streptomyces clavuligerus
Mg2+ optimal concentration 0.005 mM MgCl2 Streptomyces coelicolor

Organism

Organism UniProt Comment Textmining
Streptomyces clavuligerus Q9ZH77 NRRL3585
-
Streptomyces clavuligerus NRRL 3585 Q9ZH77 NRRL3585
-
Streptomyces coelicolor Q9RKU9 M130
-
Streptomyces coelicolor A3(2) Q9RKU9 M130
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzymes Streptomyces coelicolor
recombinant enzymes Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0012
-
-
 Streptomyces clavuligerus
0.0262
-
-
 Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces coelicolor (S)-malate + CoA
-
 ?
glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces clavuligerus (S)-malate + CoA
-
 ?
glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces coelicolor A3(2) (S)-malate + CoA
-
 ?
glyoxylate + acetyl-CoA + H2O the optimum concentration of acetyl-CoA 0.0001 mM, the optimum concentration of glyoxylate 0.001 mM Streptomyces clavuligerus NRRL 3585 (S)-malate + CoA
-
 ?

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45
-
 Streptomyces coelicolor
20 45
-
 Streptomyces clavuligerus

pH Range

pH Minimum pH Maximum Comment Organism
8 10 significant reduction of specific activity at pH 7.0 and below Streptomyces coelicolor
8 10 significant reduction of specific activity at pH 7.0 and below Streptomyces clavuligerus