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Literature summary for 2.3.3.9 extracted from
- Purification and characterization of malate synthase from the glucose-grown wood-rotting basidiomycete Fomitopsis palustris (2002), Biosci. Biotechnol. Biochem., 66, 576-581.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | above 0.02 mM | Fomitopsis palustris |  |
| ADP | 1 mM, 50% residual activity | Fomitopsis palustris | |
| AMP | 1 mM, 54% residual activity | Fomitopsis palustris | |
| ATP | 1 mM, 50% residual activity | Fomitopsis palustris | |
| Ca2+ | 5 mM, 19% residual activity | Fomitopsis palustris | |
| citrate | 1 mM, 82% residual activity | Fomitopsis palustris | |
| coenzyme A | 0.1 mM, 56% residual activity | Fomitopsis palustris | |
| D-fructose-1,6-bisphosphate | 10 mM, 60% residual activity | Fomitopsis palustris | |
| glycolate | 1 mM, 77% residual activity | Fomitopsis palustris | |
| Glyoxal | 10 mM, 70% residual activity | Fomitopsis palustris | |
| iodoacetate | 5 mM, 6% residual activity | Fomitopsis palustris | |
| K+ | 5 mM, 5% residual activity | Fomitopsis palustris | |
| Mn2+ | 5 mM, 16% residual activity | Fomitopsis palustris | |
| Na+ | 5 mM, 10% residual activity | Fomitopsis palustris | |
| oxalate | 1 mM, 43% residual activity | Fomitopsis palustris | |
| oxaloacetate | 1 mM, 92% residual activity | Fomitopsis palustris | |
| p-chloromercuribenzoate | 0.05 mM, 9% residual activity | Fomitopsis palustris | |
| phosphoenolpyruvate | 1 mM, 66% residual activity | Fomitopsis palustris | |
| pyruvate | 1 mM, 89% residual activity | Fomitopsis palustris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0022 | - |
acetyl-CoA | pH 8.0, 30°C | Fomitopsis palustris | |
| 0.045 | - |
glyoxalate | pH 8.0, 30°C | Fomitopsis palustris | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | maximal enzyme activity at 5-10 mM, Km-value 0.4 mM | Fomitopsis palustris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
8 * 65000, SDS-PAGE | Fomitopsis palustris |
| 520000 | - |
gel filtration | Fomitopsis palustris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fomitopsis palustris | - |
growth on glucose | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fomitopsis palustris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + glyoxylate + H2O | - |
Fomitopsis palustris | (S)-malate + CoA | - |
? | |
| additional information | no substrate: oxaloacetate, pyruvate, 2-oxoglutarate, glyoxal | Fomitopsis palustris | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 65000, SDS-PAGE | Fomitopsis palustris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Fomitopsis palustris |
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