Cloned (Comment) | Organism |
---|---|
GST-tagged version expressed in Escherichia coli | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
acetyl-CoA | - |
Pseudomonas aeruginosa | |
0.07 | - |
glyoxylate | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79400 | - |
x * 79400, predicted and observed (SDS-PAGE) | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione affinity chromatography, proteolytic removal of the GST tag, anion exchange chromatography | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
16.5 | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + glyoxylate + H2O | - |
Pseudomonas aeruginosa | (S)-malate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 79400, predicted and observed (SDS-PAGE) | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
malate synthase G | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37.5 | - |
- |
Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | - |
15.5% activity | Pseudomonas aeruginosa |
15 | - |
47.1% activity | Pseudomonas aeruginosa |
32.5 | 40 | more than 90% of activity | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h preincubation: 100% activity | Pseudomonas aeruginosa |
55 | - |
1 h preincubation: 47% activity | Pseudomonas aeruginosa |
70 | - |
1 h preincubation: complete loss of activity | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
below pH 6: no activity remains, outside pH 7-10 the activity decreases rapidly | Pseudomonas aeruginosa |
7 | - |
87% activity | Pseudomonas aeruginosa |
10 | - |
85% activity | Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1 | 2 | 1 h preincubation: about 5% activity | Pseudomonas aeruginosa |
5 | - |
1 h preincubation: about 5% activity | Pseudomonas aeruginosa |
6 | 7 | 1 h preincubation: 100% activity | Pseudomonas aeruginosa |
11 | - |
1 h preincubation: 90% activity | Pseudomonas aeruginosa |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
additional information | - |
1 mM pyruvate: 40% activity | Pseudomonas aeruginosa | pyruvate |