Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5'-AMP | activation | Escherichia coli | |
5'-AMP | in both directions | Escherichia coli | |
CNO- | slight activation | Escherichia coli | |
additional information | not activated by 2'-AMP, 3'-AMP, ADP, adenosine | Escherichia coli | |
additional information | not activated by ATP, cAMP | Escherichia coli | |
Na2SO4 | activation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADPglucose | competitive inhibition | Escherichia coli | |
Ag+ | strong inhibition | Escherichia coli | |
alpha-Amylose | - |
Escherichia coli | |
beta-Amylose | - |
Escherichia coli | |
Ca2+ | weak | Escherichia coli | |
CN- | - |
Escherichia coli | |
Cu2+ | 1 mM, strong inhibition | Escherichia coli | |
D-glucose | non-competitive | Escherichia coli | |
Fe2+ | weak inhibition | Escherichia coli | |
glucose 1-phosphate | above 2 mM; substrate inhibition | Escherichia coli | |
Hg2+ | 1 mM, strong inhibition | Escherichia coli | |
iodoacetamide | weak | Escherichia coli | |
iodoacetate | weak inhibition | Escherichia coli | |
K2S2O8 | - |
Escherichia coli | |
KNO3 | - |
Escherichia coli | |
additional information | not inhibited by Mg2+ and Mn2+ | Escherichia coli | |
N-ethylmaleimide | weak | Escherichia coli | |
p-chloromercuribenzoate | 1 mM, 55% inhibition | Escherichia coli | |
TDPglucose | competitive inhibition | Escherichia coli | |
UDPglucose | - |
Escherichia coli | |
Zn2+ | strong inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.46% glycogen, with AMP, 0.67% glycogen, without AMP | Escherichia coli | |
1 | - |
Glucose-1-phosphate | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not activated by Cl- | Escherichia coli | |
NaF | activation, maximal at 200 mM | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
K-12 | - |
Purification (Comment) | Organism |
---|---|
pH 5.3, protamine sulfate, ammonium sulfate, DEAE-cellulose, Sephadex G-200 | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.118 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + glucose 1-phosphate | - |
Escherichia coli | glycogen + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | favoured reaction | Escherichia coli | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | polyglucose primer required | Escherichia coli | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | catalyzes incorporation of glucose into alpha-1,4-glucosidic linkage on exterior chains of primer, glycogen synthesis is preferred | Escherichia coli | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 6.9 | glycogen | Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | approx. half-maximal activity at pH 6.0 and 7.5 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | requirement | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
ADP-glucose | - |
Escherichia coli | |
1 | - |
TDP-glucose | - |
Escherichia coli | |
2 | - |
UDP-glucose | - |
Escherichia coli | |
2.5 | - |
glucose | - |
Escherichia coli |