Literature summary for 2.4.1.1 extracted from

Thomas, D.A.; Wright, B.E.
Glycogen phosphorylase in Dictyostelium discoideum. I. Purification and properties of the enzyme (1976), J. Biol. Chem., 254, 1253-1257.

No PubMed abstract available

Search for more literature for 2.4.1.1

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	not activated by ATP, cAMP	Dictyostelium discoideum
additional information	not activated by ATP-regenerating systems	Dictyostelium discoideum

Inhibitors

Inhibitors	Comment	Organism	Structure
ADPglucose	competitive inhibition	Dictyostelium discoideum
GDPglucose	0.05 mM, 50% inhibition, competitive inhibition of phosphorolysis	Dictyostelium discoideum
glucose 1-phosphate	kinetics	Dictyostelium discoideum
UDPglucose	kinetics	Dictyostelium discoideum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	5 mg/ml, oyster glycogen	Dictyostelium discoideum
3.6	-	phosphate	-	Dictyostelium discoideum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
95000	-	2 * 95000	Dictyostelium discoideum

Organism

Organism	UniProt	Comment	Textmining
Dictyostelium discoideum	-	slime mould, strain NC-4	-

Purification (Commentary)

Purification (Comment)	Organism
Streptomycin sulfate, ammonium sulfate, heat, DEAE-cellulose, Sepharose 2B, preparative gel electrophoresis	Dictyostelium discoideum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
7.14	-	-	Dictyostelium discoideum

Storage Stability

Storage Stability	Organism
glycerol stabilizes purified enzyme during storage	Dictyostelium discoideum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
glycogen + glucose 1-phosphate	glycogen from: oyster	Dictyostelium discoideum	glycogen + phosphate	-	r
[(1->4)-alpha-D-glucosyl]n + phosphate	-	Dictyostelium discoideum	[(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 95000	Dictyostelium discoideum

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.1	-	glucose 1-phosphate	-	Dictyostelium discoideum

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Release 2023.1 (January 2023)