Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | activation | Saccharomyces cerevisiae | |
phosphate | yeast and mammalian enzymes are activated by covalent phosphorylation, phosphorylase kinase | Saccharomyces cerevisiae | |
phosphate | phosphorylation site: a single Thr-residue in the N-terminal region | Saccharomyces cerevisiae | |
Phosphorylase kinase | activation | Saccharomyces cerevisiae | |
Phosphorylase kinase | yeast and mammalian enzymes are activated by covalent phosphorylation of a single Thr-residue in the N-terminal region | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion, crystals diffract to 2.4 A resolution | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
glucose 6-phosphate | above 5 mM | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
harbouring plasmid Yep24::GPH1 | - |
Saccharomyces cerevisiae PH5-3 | - |
harbouring plasmid Yep24::GPH1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | activation by phosphorylation | Saccharomyces cerevisiae |
side-chain modification | phosphorylation site: a single Thr-residue in the N-terminal region | Saccharomyces cerevisiae |
side-chain modification | yeast and mammalian enzymes are activated by covalent phosphorylation, phosphorylase kinase | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.7 | - |
non-phosphorylated enzyme | Saccharomyces cerevisiae |
85 | 90 | phosphorylated enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + glucose 1-phosphate | favoured reaction | Saccharomyces cerevisiae | glycogen + phosphate | - |
r | |
glycogen + glucose 1-phosphate | favoured reaction | Saccharomyces cerevisiae PH5-3 | glycogen + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Saccharomyces cerevisiae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Saccharomyces cerevisiae PH5-3 | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |