Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | activation | Saccharomyces cerevisiae | |
phosphate | phosphorylation site: a single Thr-residue in the N-terminal region | Saccharomyces cerevisiae | |
Phosphorylase kinase | activation | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 10 mM, 55% inhibition, competitive to glucose 1-phosphate | Saccharomyces cerevisiae | |
Caffeine | weak | Saccharomyces cerevisiae | |
Cu2+ | 4 mM, more than 50% inhibition | Saccharomyces cerevisiae | |
cyclodextrin | alpha- or beta-, not gamma-cyclodextrin; i.e. structure analogue of alpha-1,4-linked starch molecule | Saccharomyces cerevisiae | |
glucose 6-phosphate | 5 mM, 54% inhibition | Saccharomyces cerevisiae | |
Hg2+ | 4 mM, more than 50% inhibition | Saccharomyces cerevisiae | |
additional information | not inhibited by IMP | Saccharomyces cerevisiae | |
NaF | 200 mM, complete inhibition | Saccharomyces cerevisiae | |
UDPglucose | 5 mM, 42% inhibition | Saccharomyces cerevisiae | |
Zn2+ | 4 mM, more than 50% inhibition | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
2 * 100000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | activation by phosphorylation | Saccharomyces cerevisiae |
side-chain modification | phosphorylation site: a single Thr-residue in the N-terminal region | Saccharomyces cerevisiae |
Purification (Comment) | Organism |
---|---|
Streptomycin, 33% ethanol, DEAE-Sephadex, glycogen-Sepharose | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42.3 | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + glucose 1-phosphate | - |
Saccharomyces cerevisiae | glycogen + phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Saccharomyces cerevisiae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 100000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Saccharomyces cerevisiae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
stable below | Saccharomyces cerevisiae |
40 | 50 | inactivation | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
- |
Saccharomyces cerevisiae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | stable | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
9.3 | - |
AMP | - |
Saccharomyces cerevisiae |