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Literature summary for 2.4.1.1 extracted from
- Glycogen phosphorylase from flight muscle of the hawk moth, Manduca sexta: purification and properties of three interconvertible forms and the effect of flight on their interconversion (1994), J. Comp. Physiol. B, 164, 261-271.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 5'-AMP | 1 mM, 200fold activation of phosphorylase b, completely abolished by caffeine, half-maximal activation of phosphorylases b, ab and a at 0.466, 0.0154 and 0.001 mM AMP respectively | Manduca sexta |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Manduca sexta | |
| Caffeine | 5 mM, complete inhibition of phosphorylase b, strong inhibition of phosphorylases ab and a | Manduca sexta | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
glycogen | phosphorylase a | Manduca sexta | |
| 0.55 | - |
glycogen | phosphorylase ab | Manduca sexta | |
| 2.01 | - |
phosphate | phosphorylase a | Manduca sexta | |
| 3.03 | - |
phosphate | phosphorylase ab | Manduca sexta | |
| 6.04 | - |
glycogen | phosphorylase b | Manduca sexta | |
| 17.89 | - |
phosphate | phosphorylase b | Manduca sexta | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 92000 | - |
2 * 92000, phosphorylase a, ab and b, SDS-PAGE | Manduca sexta |
| 178000 | - |
phosphorylases a, ab and b, native PAGE | Manduca sexta |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Manduca sexta | - |
hawk moth | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| side-chain modification | in vitro phosphorylation of phosphorylase b with phosphorylase kinase from rabbit muscle | Manduca sexta |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| phosphorylases b, ab and a, DEAE-Sephacel, AMP-Sepharose | Manduca sexta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| muscle | flight muscle | Manduca sexta | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.11 | - |
phosphorylase b | Manduca sexta |
| 10.08 | - |
phosphorylase ab | Manduca sexta |
| 12.12 | - |
phosphorylase a | Manduca sexta |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycogen + glucose 1-phosphate | - |
Manduca sexta | glycogen + phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 92000, phosphorylase a, ab and b, SDS-PAGE | Manduca sexta |
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