Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 50-300 mM, weak inhibition | Corynebacterium callunae | |
ADP | 5 mM, 8% inhibition of glucan phosphorolysis | Corynebacterium callunae | |
AMP | 1-20 mM, weak inhibition | Corynebacterium callunae | |
ATP | 5 mM, 10% inhibition of glucan phosphorolysis | Corynebacterium callunae | |
D-glucono-delta-lactone | 0.05 mM, 90% inhibition of glucan phosphorolysis | Corynebacterium callunae | |
D-glucose | 50 mM, 30% inhibition of glucan phosphorolysis | Corynebacterium callunae | |
molybdate | 5 mM, more than 50% inhibition of glucan phosphorolysis | Corynebacterium callunae | |
Na2SO4 | 50-300 mM, weak inhibition | Corynebacterium callunae | |
vanadate | 5 mM, more than 50% inhibition of glucan phosphorolysis | Corynebacterium callunae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
starch | phosphorolysis | Corynebacterium callunae | |
0.27 | - |
glycogen DP14 | phosphorolysis | Corynebacterium callunae | |
0.47 | - |
starch DP24 | synthesis | Corynebacterium callunae | |
0.5 | - |
dextrin DP11 | phosphorolysis | Corynebacterium callunae | |
1.04 | - |
glucose 1-phosphate | dextrin DP11 synthesis | Corynebacterium callunae | |
1.18 | - |
phosphate | dextrin DP9 phosphorolysis | Corynebacterium callunae | |
1.22 | - |
dextrin DP9 | phosphorolysis | Corynebacterium callunae | |
2.73 | - |
maltopentaose | phosphorolysis | Corynebacterium callunae | |
3.15 | - |
maltohexaose | phosphorolysis | Corynebacterium callunae | |
3.72 | - |
maltoheptaose | phosphorolysis | Corynebacterium callunae | |
4.32 | - |
glycogen DP14 | synthesis | Corynebacterium callunae | |
22.7 | - |
arsenate | maltohexaose arsenolysis | Corynebacterium callunae | |
39.86 | - |
maltoheptaose | synthesis | Corynebacterium callunae | |
46.48 | - |
dextrin DP11 | synthesis | Corynebacterium callunae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
2 * 88000, SDS-PAGE | Corynebacterium callunae |
176000 | - |
gel filtration | Corynebacterium callunae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium callunae | - |
DSM 20147 | - |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose, maltodextrin-Sepharose 6B, methyl-column | Corynebacterium callunae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | sequential mechanism | Corynebacterium callunae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.53 | - |
- |
Corynebacterium callunae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(dextrin)n-1 + glucose 1-phosphate | - |
Corynebacterium callunae | (dextrin)n + phosphate | - |
? | |
dextrin DP11 + phosphate | - |
Corynebacterium callunae | dextrin DP10 + alpha-D-glucose 1-phosphate | - |
? | |
dextrin DP9 + phosphate | - |
Corynebacterium callunae | dextrin DP8 + alpha-D-glucose 1-phosphate | - |
? | |
glycogen + glucose 1-phosphate | - |
Corynebacterium callunae | glycogen + phosphate | - |
r | |
glycogen DP14 + phosphate | - |
Corynebacterium callunae | glycogen DP13 + alpha-D-glucose 1-phosphate | - |
r | |
maltoheptaose + glucose 1-phosphate | - |
Corynebacterium callunae | maltooctaose + phosphate | - |
r | |
maltohexaose + arsenate | - |
Corynebacterium callunae | maltopentaose + glucose 1-arsenate | - |
? | |
maltohexaose + glucose 1-phosphate | - |
Corynebacterium callunae | maltoheptaose + phosphate | - |
r | |
maltopentaose + glucose 1-phosphate | - |
Corynebacterium callunae | maltohexaose + phosphate | - |
r | |
maltotetraose + glucose 1-phosphate | - |
Corynebacterium callunae | maltopentaose + phosphate | - |
ir | |
maltotriose + glucose 1-phosphate | - |
Corynebacterium callunae | maltotetraose + phosphate | - |
r | |
starch + glucose 1-phosphate | - |
Corynebacterium callunae | starch + phosphate | - |
r | |
starch DP24 + phosphate | - |
Corynebacterium callunae | starch DP23 + alpha-D-glucose 1-phosphate | - |
r | |
[(1->4)-alpha-D-glucosyl]n + phosphate | - |
Corynebacterium callunae | [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 88000, SDS-PAGE | Corynebacterium callunae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
glucan phosphorolysis | Corynebacterium callunae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
arsenate | maltohexaose arsenolysis | Corynebacterium callunae | |
33.8 | - |
maltopentaose | phosphorolysis | Corynebacterium callunae | |
34.5 | - |
glycogen DP14 | phosphorolysis | Corynebacterium callunae | |
38.9 | - |
maltohexaose | phosphorolysis | Corynebacterium callunae | |
41.3 | - |
maltoheptaose | phosphorolysis | Corynebacterium callunae | |
47.3 | - |
dextrin DP9 | phosphorolysis | Corynebacterium callunae | |
50 | - |
dextrin DP11 | phosphorolysis | Corynebacterium callunae | |
50.5 | - |
phosphate | dextrin DP9 phosphorolysis | Corynebacterium callunae | |
69.9 | - |
starch | phosphorolysis | Corynebacterium callunae | |
131 | - |
glucose 1-phosphate | dextrin DP11 synthesis | Corynebacterium callunae | |
187 | - |
dextrin DP11 | synthesis | Corynebacterium callunae | |
283 | - |
glycogen DP14 | synthesis | Corynebacterium callunae | |
300 | - |
starch DP24 | synthesis | Corynebacterium callunae | |
312 | - |
maltoheptaose | synthesis | Corynebacterium callunae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.7 | 6.1 | glucan synthesis | Corynebacterium callunae |
7 | 7.2 | glucan phosphorolysis | Corynebacterium callunae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | 0.87 mol/subunit | Corynebacterium callunae |