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Literature summary for 2.4.1.1 extracted from

  • Weinhausel, A.; Griessler, R.; Krebs, A.; Zipper, P.; Haltrich, D.; Kulbe, K.D.; Nidetzky, B.
    alpha-1,4-D-Glucan phosphorylase of gram-positive Corynebacterium callunae: isolation, biochemical properties and molecular shape of the enzyme from solution X-ray scattering (1997), Biochem. J., 326, 773-783.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 50-300 mM, weak inhibition Corynebacterium callunae
ADP 5 mM, 8% inhibition of glucan phosphorolysis Corynebacterium callunae
AMP 1-20 mM, weak inhibition Corynebacterium callunae
ATP 5 mM, 10% inhibition of glucan phosphorolysis Corynebacterium callunae
D-glucono-delta-lactone 0.05 mM, 90% inhibition of glucan phosphorolysis Corynebacterium callunae
D-glucose 50 mM, 30% inhibition of glucan phosphorolysis Corynebacterium callunae
molybdate 5 mM, more than 50% inhibition of glucan phosphorolysis Corynebacterium callunae
Na2SO4 50-300 mM, weak inhibition Corynebacterium callunae
vanadate 5 mM, more than 50% inhibition of glucan phosphorolysis Corynebacterium callunae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
starch phosphorolysis Corynebacterium callunae
0.27
-
glycogen DP14 phosphorolysis Corynebacterium callunae
0.47
-
starch DP24 synthesis Corynebacterium callunae
0.5
-
dextrin DP11 phosphorolysis Corynebacterium callunae
1.04
-
glucose 1-phosphate dextrin DP11 synthesis Corynebacterium callunae
1.18
-
phosphate dextrin DP9 phosphorolysis Corynebacterium callunae
1.22
-
dextrin DP9 phosphorolysis Corynebacterium callunae
2.73
-
maltopentaose phosphorolysis Corynebacterium callunae
3.15
-
maltohexaose phosphorolysis Corynebacterium callunae
3.72
-
maltoheptaose phosphorolysis Corynebacterium callunae
4.32
-
glycogen DP14 synthesis Corynebacterium callunae
22.7
-
arsenate maltohexaose arsenolysis Corynebacterium callunae
39.86
-
maltoheptaose synthesis Corynebacterium callunae
46.48
-
dextrin DP11 synthesis Corynebacterium callunae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
88000
-
2 * 88000, SDS-PAGE Corynebacterium callunae
176000
-
gel filtration Corynebacterium callunae

Organism

Organism UniProt Comment Textmining
Corynebacterium callunae
-
DSM 20147
-

Purification (Commentary)

Purification (Comment) Organism
phenyl-Sepharose, maltodextrin-Sepharose 6B, methyl-column Corynebacterium callunae

Reaction

Reaction Comment Organism Reaction ID
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate sequential mechanism Corynebacterium callunae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
33.53
-
-
Corynebacterium callunae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(dextrin)n-1 + glucose 1-phosphate
-
Corynebacterium callunae (dextrin)n + phosphate
-
?
dextrin DP11 + phosphate
-
Corynebacterium callunae dextrin DP10 + alpha-D-glucose 1-phosphate
-
?
dextrin DP9 + phosphate
-
Corynebacterium callunae dextrin DP8 + alpha-D-glucose 1-phosphate
-
?
glycogen + glucose 1-phosphate
-
Corynebacterium callunae glycogen + phosphate
-
r
glycogen DP14 + phosphate
-
Corynebacterium callunae glycogen DP13 + alpha-D-glucose 1-phosphate
-
r
maltoheptaose + glucose 1-phosphate
-
Corynebacterium callunae maltooctaose + phosphate
-
r
maltohexaose + arsenate
-
Corynebacterium callunae maltopentaose + glucose 1-arsenate
-
?
maltohexaose + glucose 1-phosphate
-
Corynebacterium callunae maltoheptaose + phosphate
-
r
maltopentaose + glucose 1-phosphate
-
Corynebacterium callunae maltohexaose + phosphate
-
r
maltotetraose + glucose 1-phosphate
-
Corynebacterium callunae maltopentaose + phosphate
-
ir
maltotriose + glucose 1-phosphate
-
Corynebacterium callunae maltotetraose + phosphate
-
r
starch + glucose 1-phosphate
-
Corynebacterium callunae starch + phosphate
-
r
starch DP24 + phosphate
-
Corynebacterium callunae starch DP23 + alpha-D-glucose 1-phosphate
-
r
[(1->4)-alpha-D-glucosyl]n + phosphate
-
Corynebacterium callunae [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 88000, SDS-PAGE Corynebacterium callunae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
glucan phosphorolysis Corynebacterium callunae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.9
-
arsenate maltohexaose arsenolysis Corynebacterium callunae
33.8
-
maltopentaose phosphorolysis Corynebacterium callunae
34.5
-
glycogen DP14 phosphorolysis Corynebacterium callunae
38.9
-
maltohexaose phosphorolysis Corynebacterium callunae
41.3
-
maltoheptaose phosphorolysis Corynebacterium callunae
47.3
-
dextrin DP9 phosphorolysis Corynebacterium callunae
50
-
dextrin DP11 phosphorolysis Corynebacterium callunae
50.5
-
phosphate dextrin DP9 phosphorolysis Corynebacterium callunae
69.9
-
starch phosphorolysis Corynebacterium callunae
131
-
glucose 1-phosphate dextrin DP11 synthesis Corynebacterium callunae
187
-
dextrin DP11 synthesis Corynebacterium callunae
283
-
glycogen DP14 synthesis Corynebacterium callunae
300
-
starch DP24 synthesis Corynebacterium callunae
312
-
maltoheptaose synthesis Corynebacterium callunae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7 6.1 glucan synthesis Corynebacterium callunae
7 7.2 glucan phosphorolysis Corynebacterium callunae

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate 0.87 mol/subunit Corynebacterium callunae