Crystallization (Comment) | Organism |
---|---|
enzyme complexed with inhibitor beta-D-glucopyranosyl bismethoxyphosphoramidate by soaking of native enzyme crystals in 150 mM inhibitor 7.5 h prior to diffraction data collection, X-ray diffraction structure determination and analysis at 1.83 A resolution, modeling | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-D-glucopyranosyl bismethoxyphosphoramidate | weak competitive inhibitor, binds at the catalytic site and induces conformational changes in the vicinity of the site, inhibition mechanism, overview | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P00489 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
[(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | catalytic site structure | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrate is glycogen | Oryctolagus cuniculus | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
glycogen phosphorylase b | - |
Oryctolagus cuniculus |
GP | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at, synthesis reaction | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at, synthesis reaction | Oryctolagus cuniculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
beta-D-glucopyranosyl bismethoxyphosphoramidate | pH 6.8, 30°C, versus alpha-D-glucose 1-phosphate, synthesis reaction | Oryctolagus cuniculus |