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Literature summary for 2.4.1.1 extracted from
- Purification and characterization of a cytosolic starch phosphorylase from etiolated rice seedlings (2004), Bot. Bull. Acad. Sin., 45, 187-196.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight activation | Oryza sativa |  |
| DTT | slight activation | Oryza sativa | |
| glutathione | slight activation | Oryza sativa | |
| L-cysteine | slight activation | Oryza sativa | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP-glucose | strong inhibition | Oryza sativa | |
| AMP | slight inhibition | Oryza sativa | |
| Cyclodextrins | - |
Oryza sativa | |
| D-fructose 6-phosphate | strong inhibition | Oryza sativa | |
| D-mannose 1-phosphate | strong inhibition | Oryza sativa | |
| IMP | slight inhibition | Oryza sativa | |
| additional information | no or poor inhibition by ribose 5-phosphate, glucose 6-phosphate, fructose-1,6-bisphosphate, ADP and UDP | Oryza sativa | |
| UDP-glucose | strong inhibition | Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0046 | - |
amylopectin | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
| 0.0106 | - |
soluble starch | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
| 0.0138 | - |
glycogen | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
| 0.0261 | - |
soluble starch | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
| 0.0418 | - |
Dextrin | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
| 2.1 | - |
alpha-D-glucose 1-phosphate | pH 5.5, 37°C, synthesis reaction | Oryza sativa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Oryza sativa | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by Na+, K+, Mg2+, Ca2+ | Oryza sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-alpha-D-glucosyl)n + phosphate | Oryza sativa | polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | - |
cv. Tainong 67, enzyme Pho1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from seedlings, 299fold to homogeneity by protamine sulfate and ammonium sulfate fractionation, anion exchange chromatography, and dextrin affinity chromatography | Oryza sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | etiolated, low enzyme content | Oryza sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity | Oryza sativa |
| 1.8 | - |
purified enzyme | Oryza sativa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| purified enzyme in 50 mM imidazole, pH 7.0, 1 mM EDTA, 1 mM DTT, at 4°C, at least 2 weeks stable | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrate is starch, phosphorolysis is the physiologic reaction of the starch degrading enzyme | Oryza sativa | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| (1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrates are soluble starch and Zulkowsky soluble starch, amylopectin, glycogen, and dextrin, amyylopectin is the best substrate | Oryza sativa | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| amylopectin + phosphate | - |
Oryza sativa | amylopectin + D-glucose-1-phosphate | - |
? | |
| dextrin + alpha-D-glucose 1-phosphate | - |
Oryza sativa | dextrin + phosphate | - |
? | |
| glycogen + phosphate | - |
Oryza sativa | alpha-D-glucan + alpha-D-glucose 1-phosphate | - |
r | |
| maltodecaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | ? + phosphate | - |
r | |
| maltoheptaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltooctaose + phosphate | - |
r | |
| maltohexaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltoheptaose + phosphate | - |
r | |
| maltononaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltodecaose + phosphate | - |
r | |
| maltooctaose + alpha-D-glucose 1-phosphate | low activity | Oryza sativa | maltononaose + phosphate | - |
r | |
| maltopentaose + alpha-D-glucose 1-phosphate | - |
Oryza sativa | maltohexaose + phosphate | - |
r | |
| maltotetraose + alpha-D-glucose 1-phosphate | - |
Oryza sativa | maltopentaose + phosphate | - |
r | |
| additional information | substrate specificity, no or nearly no activity with glucose, maltose, and maltotriose, overview | Oryza sativa | ? | - |
? | |
| soluble starch + phosphate | - |
Oryza sativa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-glucan phosphorylase | - |
Oryza sativa |
| PHO | - |
Oryza sativa |
| SP | - |
Oryza sativa |
| starch phosphorylase | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Oryza sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
synthesis reaction | Oryza sativa |
| 6 | - |
assay at, phosphorolysis reaction | Oryza sativa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
most stable at | Oryza sativa |
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