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Literature summary for 2.4.1.1 extracted from
- Characterization of a hyperthermostable glycogen phosphorylase from Aquifex aeolicus expressed in Escherichia coli (2003), J. Mol. Catal. B, 22, 173-180.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene glgP, DNA sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3) | Aquifex aeolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics, forward and reverse reaction, recombinant enzyme | Aquifex aeolicus | |
| 0.17 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus |  |
| 0.18 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
| 0.19 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
| 0.28 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 0.3 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
| 0.3 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 0.38 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 0.55 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 1.8 | - |
maltotriose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
| 81981 | - |
x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-alpha-D-glucosyl)n + phosphate | Aquifex aeolicus | polysaccharide substrate is glycogen, mobilization of glucose 1-phosphate as readily useable energy source | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O66932 | gene glgP | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli 6.3fold to homogeneity by heat treatment at 70°C and nickel affinity chromatography | Aquifex aeolicus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| [(1->4)-alpha-D-glucosyl]n + phosphate = [(1->4)-alpha-D-glucosyl]n-1 + alpha-D-glucose 1-phosphate | sequential random bi bi mechanism | Aquifex aeolicus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
purified recombinant enzyme | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrate is glycogen, mobilization of glucose 1-phosphate as readily useable energy source | Aquifex aeolicus | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| (1,4-alpha-D-glucosyl)n + phosphate | polysaccharide substrates are glycogen and starch, phosphorolysis is the preferred reaction | Aquifex aeolicus | (1,4-alpha-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
r | |
| maltoheptaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltooctaose + phosphate | - |
r | |
| maltohexaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltoheptaose + phosphate | - |
r | |
| maltopentaose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltohexaose + phosphate | - |
r | |
| maltotetraose + alpha-D-glucose 1-phosphate | - |
Aquifex aeolicus | maltopentaose + phosphate | - |
r | |
| maltotriose + alpha-D-glucose 1-phosphate | maltotriose is the smallest substrate accepted for the synthetic reaction direction, maltotetraose is the smallest substrate accepted for the phosphorolytic reaction direction | Aquifex aeolicus | maltotetraose + phosphate | - |
r | |
| additional information | maltotriose is the smallest substrate accepted for the synthetic reaction direction, maltotetraose is the smallest substrate accepted for the phosphorolytic reaction direction | Aquifex aeolicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, recombinant His-tagged enzyme, SDS-PAGE, x * 81981, sequence calculation | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glycogen phosphorylase | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 100 | - |
recombinant enzyme | Aquifex aeolicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
84% remaining activity after 30 min, recombinant enzyme | Aquifex aeolicus |
| 100 | - |
66% remaining activity after 30 min, recombinant enzyme | Aquifex aeolicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.76 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
| 2.2 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
| 2.3 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
| 2.7 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, phosphorolysis reaction | Aquifex aeolicus | |
| 4.1 | - |
maltohexaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 4.4 | - |
maltoheptaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 5.4 | - |
maltopentaose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 6 | - |
maltotetraose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
| 6.8 | - |
maltotriose | pH 6.5, 37°C, recombinant enzyme, synthesis reaction | Aquifex aeolicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
recombinant enzyme | Aquifex aeolicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | recombinant enzyme, stable, 61% remaining activity at 70°C and pH 10.4 | Aquifex aeolicus |
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