Protein Variants | Comment | Organism |
---|---|---|
F39L | mutant with enhanced thermostability and resistance to proteolytic degradation | Solanum tuberosum |
F39L/N135S | mutant with enhanced thermostability and resistance to proteolytic degradation | Solanum tuberosum |
F39L/N135S/T706I | mutant with enhanced thermostability and resistance to proteolytic degradation | Solanum tuberosum |
F39L/T706I | mutant with enhanced thermostability and resistance to proteolytic degradation | Solanum tuberosum |
N135S | mutant with enhanced thermostability | Solanum tuberosum |
N135S/T706I | mutant with enhanced thermostability and resistance to proteolytic degradation | Solanum tuberosum |
T706I | mutant with enhanced thermostability and resistance to proteolytic degradation | Solanum tuberosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
D-glucose-1-phosphate | mutant T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.48 | - |
D-glucose-1-phosphate | mutant F39L/N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.56 | - |
D-glucose-1-phosphate | mutant N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.57 | - |
D-glucose-1-phosphate | wild-type, 37°C, pH 5.5 | Solanum tuberosum | |
0.59 | - |
D-glucose-1-phosphate | mutant N135S, 37°C, pH 5.5 | Solanum tuberosum | |
0.62 | - |
D-glucose-1-phosphate | mutant F39L/N135S, 37°C, pH 5.5 | Solanum tuberosum | |
0.76 | - |
D-glucose-1-phosphate | mutant F39L/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.96 | - |
D-glucose-1-phosphate | mutant F39L, 37°C, pH 5.5 | Solanum tuberosum | |
1.54 | - |
maltotetraose | mutant F39L/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
1.87 | - |
maltotetraose | mutant T706I, 37°C, pH 5.5 | Solanum tuberosum | |
1.96 | - |
maltotetraose | mutant N135S, 37°C, pH 5.5 | Solanum tuberosum | |
2 | - |
maltotetraose | mutant F39L, 37°C, pH 5.5 | Solanum tuberosum | |
2 | - |
maltotetraose | mutant F39L/N135S, 37°C, pH 5.5 | Solanum tuberosum | |
2.11 | - |
maltotetraose | wild-type, 37°C, pH 5.5 | Solanum tuberosum | |
2.47 | - |
maltotetraose | mutant N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
2.57 | - |
maltotetraose | mutant F39L/N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
105000 | - |
2 * 105000, SDS-PAGE | Solanum tuberosum |
210000 | - |
PAGE | Solanum tuberosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum tuberosum | - |
type L alpha-glucan phosphorylase | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltotetraose + D-glucose-1-phosphate | - |
Solanum tuberosum | maltopentaose + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 105000, SDS-PAGE | Solanum tuberosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
wild-type, complete loss of activity within 20 min. Mutants F39L, N135S and T706I retain about 50% of activity after 20 min at 60°C. Mutant F39L/N135S/T706I retains more than 95% of its activity after 60 min at 60°C | Solanum tuberosum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
D-glucose-1-phosphate | wild-type, 37°C, pH 5.5 | Solanum tuberosum | |
0.0017 | - |
maltotetraose | mutant F39L, 37°C, pH 5.5 | Solanum tuberosum | |
0.0019 | - |
D-glucose-1-phosphate | mutant N135S, 37°C, pH 5.5 | Solanum tuberosum | |
0.002 | - |
maltotetraose | mutant N135S, 37°C, pH 5.5 | Solanum tuberosum | |
0.0023 | - |
D-glucose-1-phosphate | mutant F39L, 37°C, pH 5.5 | Solanum tuberosum | |
0.0026 | - |
D-glucose-1-phosphate | mutant T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.0027 | - |
maltotetraose | mutant F39L/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.0029 | - |
maltotetraose | mutant T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.0036 | - |
maltotetraose | mutant F39L/N135S, 37°C, pH 5.5 | Solanum tuberosum | |
0.0036 | - |
D-glucose-1-phosphate | mutant F39L/N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.0037 | - |
maltotetraose | mutant F39L/N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.0037 | - |
maltotetraose | wild-type, 37°C, pH 5.5 | Solanum tuberosum | |
0.0041 | - |
D-glucose-1-phosphate | mutant N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.0042 | - |
D-glucose-1-phosphate | mutant F39L/N135S, 37°C, pH 5.5 | Solanum tuberosum | |
0.0044 | - |
maltotetraose | mutant N135S/T706I, 37°C, pH 5.5 | Solanum tuberosum | |
0.0049 | - |
D-glucose-1-phosphate | mutant F39L/T706I, 37°C, pH 5.5 | Solanum tuberosum |