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Literature summary for 2.4.1.1 extracted from

  • Yanase, M.; Takata, H.; Fujii, K.; Takaha, T.; Kuriki, T.
    Cumulative effect of amino acid replacements results in enhanced thermostability of potato type L alpha-glucan phosphorylase (2005), Appl. Environ. Microbiol., 71, 5433-5439.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
F39L mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
F39L/N135S mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
F39L/N135S/T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
F39L/T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
N135S mutant with enhanced thermostability Solanum tuberosum
N135S/T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum
T706I mutant with enhanced thermostability and resistance to proteolytic degradation Solanum tuberosum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.33
-
D-glucose-1-phosphate mutant T706I, 37°C, pH 5.5 Solanum tuberosum
0.48
-
D-glucose-1-phosphate mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
0.56
-
D-glucose-1-phosphate mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
0.57
-
D-glucose-1-phosphate wild-type, 37°C, pH 5.5 Solanum tuberosum
0.59
-
D-glucose-1-phosphate mutant N135S, 37°C, pH 5.5 Solanum tuberosum
0.62
-
D-glucose-1-phosphate mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
0.76
-
D-glucose-1-phosphate mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum
0.96
-
D-glucose-1-phosphate mutant F39L, 37°C, pH 5.5 Solanum tuberosum
1.54
-
maltotetraose mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum
1.87
-
maltotetraose mutant T706I, 37°C, pH 5.5 Solanum tuberosum
1.96
-
maltotetraose mutant N135S, 37°C, pH 5.5 Solanum tuberosum
2
-
maltotetraose mutant F39L, 37°C, pH 5.5 Solanum tuberosum
2
-
maltotetraose mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
2.11
-
maltotetraose wild-type, 37°C, pH 5.5 Solanum tuberosum
2.47
-
maltotetraose mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
2.57
-
maltotetraose mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
2 * 105000, SDS-PAGE Solanum tuberosum
210000
-
PAGE Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
type L alpha-glucan phosphorylase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltotetraose + D-glucose-1-phosphate
-
Solanum tuberosum maltopentaose + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 105000, SDS-PAGE Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
wild-type, complete loss of activity within 20 min. Mutants F39L, N135S and T706I retain about 50% of activity after 20 min at 60°C. Mutant F39L/N135S/T706I retains more than 95% of its activity after 60 min at 60°C Solanum tuberosum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.001
-
D-glucose-1-phosphate wild-type, 37°C, pH 5.5 Solanum tuberosum
0.0017
-
maltotetraose mutant F39L, 37°C, pH 5.5 Solanum tuberosum
0.0019
-
D-glucose-1-phosphate mutant N135S, 37°C, pH 5.5 Solanum tuberosum
0.002
-
maltotetraose mutant N135S, 37°C, pH 5.5 Solanum tuberosum
0.0023
-
D-glucose-1-phosphate mutant F39L, 37°C, pH 5.5 Solanum tuberosum
0.0026
-
D-glucose-1-phosphate mutant T706I, 37°C, pH 5.5 Solanum tuberosum
0.0027
-
maltotetraose mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum
0.0029
-
maltotetraose mutant T706I, 37°C, pH 5.5 Solanum tuberosum
0.0036
-
maltotetraose mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
0.0036
-
D-glucose-1-phosphate mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
0.0037
-
maltotetraose mutant F39L/N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
0.0037
-
maltotetraose wild-type, 37°C, pH 5.5 Solanum tuberosum
0.0041
-
D-glucose-1-phosphate mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
0.0042
-
D-glucose-1-phosphate mutant F39L/N135S, 37°C, pH 5.5 Solanum tuberosum
0.0044
-
maltotetraose mutant N135S/T706I, 37°C, pH 5.5 Solanum tuberosum
0.0049
-
D-glucose-1-phosphate mutant F39L/T706I, 37°C, pH 5.5 Solanum tuberosum