Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme mutants display a significant decrease in amounts of starch during storage that correlates with the accumulation of abnormally shaped granules containing a modified amylopekti structure and a high amylose content | Chlamydomonas reinhardtii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-glucose | maximal inhibition achieved is 70%; maximal inhibition achieved is 95% | Chlamydomonas reinhardtii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C | Chlamydomonas reinhardtii | |
0.68 | - |
alpha-D-glucose 1-phosphate | pH 7.0, 30°C | Chlamydomonas reinhardtii | |
1.6 | - |
phosphate | pH 7.0, 30°C | Chlamydomonas reinhardtii | |
1.9 | - |
phosphate | pH 7.0, 30°C | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Chlamydomonas reinhardtii | 9536 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated | Chlamydomonas reinhardtii |
94000 | - |
2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated | Chlamydomonas reinhardtii |
106000 | - |
2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme | Chlamydomonas reinhardtii |
108000 | - |
2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme | Chlamydomonas reinhardtii |
182000 | - |
gel filtration | Chlamydomonas reinhardtii |
214000 | - |
gel filtration | Chlamydomonas reinhardtii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Chlamydomonas reinhardtii | enzyme is involved in storage starch synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | Q2VA40 | isoform PhoA | - |
Chlamydomonas reinhardtii | Q2VA41 | isoform PhoB, encoded by STA4 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | - |
Chlamydomonas reinhardtii |
proteolytic modification | cleavage of a 55 amino acid transit peptide | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(maltooligosaccaride)n-1 + alpha-D-glucose 1-phosphate | - |
Chlamydomonas reinhardtii | (maltooligosaccharide)n + phosphate | - |
r | |
(maltooligosaccaride)n-1 + alpha-D-glucose 1-phosphate | preferred substrate | Chlamydomonas reinhardtii | (maltooligosaccharide)n + phosphate | - |
r | |
glycogen + alpha-D-glucose 1-phosphate | - |
Chlamydomonas reinhardtii | glycogen + phosphate | - |
r | |
additional information | enzyme is involved in storage starch synthesis | Chlamydomonas reinhardtii | ? | - |
? | |
starch + alpha-D-glucose 1-phosphate | - |
Chlamydomonas reinhardtii | starch + phosphate | - |
r | |
starch + alpha-D-glucose 1-phosphate | preferred substrate | Chlamydomonas reinhardtii | starch + phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 106000, isoform PhoB, SDS-PAGE, 2 * 108000, calculated for mature enzyme | Chlamydomonas reinhardtii |
dimer | 2 * 91000, SDS-PAGE, 2 * 94000, mature enzyme, calculated | Chlamydomonas reinhardtii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 40 | broad | Chlamydomonas reinhardtii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
isoform PhoA | Chlamydomonas reinhardtii |
7.5 | - |
- |
Chlamydomonas reinhardtii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
ADP-glucose | presence of 1 mM orthophosphate, pH 7.0, 30°C | Chlamydomonas reinhardtii | |
1 | - |
ADP-glucose | presence of 1 mM orthophosphate, pH 7.0, 30°C | Chlamydomonas reinhardtii |