Application | Comment | Organism |
---|---|---|
synthesis | maltodextrin phosphorylase from Pyrococcus furiosus is fused with the cellulose-binding domain of Clostridium cellulovorans serving as an aggregation module. After molecular cloning of the corresponding gene fusion construct and controlled expression in Escherichia coli BL21, 83% of total maltodextrin phosphorylase activity is displayed in active inclusion bodies. These active inclusion bodies are easily isolated by nonionic detergent treatment and directly used for maltodextrin conversion to alpha-D-glucose-1-phosphate in a repetitive batch mode. Only 10% of enzyme activity is lost after ten conversion cycles at optimum conditions | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
maltodextrin phosphorylase from Pyrococcus furiosus is fused with the cellulose-binding domain of Clostridium cellulovorans serving as an aggregation module. After molecular cloning of the corresponding gene fusion construct and controlled expression in Escherichia coli BL21, 83% of total maltodextrin phosphorylase activity is displayed in active inclusion bodies | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
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Pyrococcus furiosus | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
maltodextrin phosphorylase | - |
Pyrococcus furiosus |