Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | complete inhibition at 1 mM | Bacillus amyloliquefaciens | |
dithiothreitol | complete inhibition at 1 mM | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
sucrose | - |
Bacillus subtilis | |
19 | - |
sucrose | - |
Bacillus amyloliquefaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
extracellular | - |
Bacillus amyloliquefaciens | - |
- |
membrane | associated | Bacillus subtilis | 16020 | - |
membrane | associated | Bacillus amyloliquefaciens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition | Bacillus subtilis |
additional information | - |
amino acid composition | Bacillus amyloliquefaciens |
50000 | 52000 | extracellular and membrane-associated enzyme forms, SDS-PAGE, gel filtration | Bacillus subtilis |
50000 | 52000 | extracellular and membrane-associated enzyme forms, SDS-PAGE, gel filtration | Bacillus amyloliquefaciens |
52000 | - |
1 * 52000, extracellular and membrane-associated form, SDS-PAGE | Bacillus subtilis |
52000 | - |
1 * 52000, extracellular and membrane-associated form, SDS-PAGE | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | Bacillus subtilis | activity is affected by sacU mutation | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | Bacillus amyloliquefaciens | activity is affected by sacU mutation | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | Bacillus subtilis QB127 | activity is affected by sacU mutation | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
strain ATCC 23350 | - |
Bacillus subtilis | - |
- |
- |
Bacillus subtilis QB127 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
203 | - |
purified enzyme | Bacillus subtilis |
415 | - |
purified enzyme | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | - |
Bacillus subtilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Bacillus amyloliquefaciens | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | activity is affected by sacU mutation | Bacillus subtilis | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | activity is affected by sacU mutation | Bacillus amyloliquefaciens | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | - |
Bacillus subtilis QB127 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | activity is affected by sacU mutation | Bacillus subtilis QB127 | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 52000, extracellular and membrane-associated form, SDS-PAGE | Bacillus subtilis |
monomer | 1 * 52000, extracellular and membrane-associated form, SDS-PAGE | Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Bacillus subtilis |
25 | - |
assay at | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
t1/2: 9 min | Bacillus amyloliquefaciens |
50 | - |
t1/2: 4 min | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | 6 | - |
Bacillus subtilis |
6 | 6.2 | - |
Bacillus amyloliquefaciens |