Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Hordeum vulgare |
screening of barley cDNA library, cloning, transient functional expression in Nicotiana plumbaginifolia protoplasts under control of cauliflower mosaic virus 35S promotor | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Nicotiana plumbaginifolia plants that transiently express the functional levansucrase | Hordeum vulgare |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence | Hordeum vulgare |
23000 | - |
1 * 49000 + 1 * 23000, SDS-PAGE | Hordeum vulgare |
49000 | - |
1 * 49000 + 1 * 23000, SDS-PAGE | Hordeum vulgare |
67000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | Hordeum vulgare | transgenic plants | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | Hordeum vulgare | biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
2 closely similar isoforms | - |
Hordeum vulgare | - |
cv. Express | - |
Purification (Comment) | Organism |
---|---|
partial purification | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,6-beta-D-fructosyl)n | - |
Hordeum vulgare | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | transgenic plants | Hordeum vulgare | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,6-beta-D-fructosyl)n | biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants | Hordeum vulgare | glucose + (2,6-beta-D-fructosyl)n+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 49000 + 1 * 23000, SDS-PAGE | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
6-SFT | - |
Hordeum vulgare |
sucrose:fructan 6-fructosyltransferase | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
27 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.75 | - |
assay at | Hordeum vulgare |