Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.10 extracted from

  • Sprenger, N.; Bortlik, K.; Brandt, A.; Boller, T.; Wiemken, A.
    Purification, cloning, and functional expression of sucrose:fructan 6-fructosyltransferase, a key enzyme of fructan synthesis in barley (1995), Proc. Natl. Acad. Sci. USA, 92, 11652-11656.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Hordeum vulgare
screening of barley cDNA library, cloning, transient functional expression in Nicotiana plumbaginifolia protoplasts under control of cauliflower mosaic virus 35S promotor Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic Nicotiana plumbaginifolia plants that transiently express the functional levansucrase Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid sequence Hordeum vulgare
23000
-
1 * 49000 + 1 * 23000, SDS-PAGE Hordeum vulgare
49000
-
1 * 49000 + 1 * 23000, SDS-PAGE Hordeum vulgare
67000
-
gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n Hordeum vulgare transgenic plants glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + (2,6-beta-D-fructosyl)n Hordeum vulgare biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants glucose + (2,6-beta-D-fructosyl)n+1
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
2 closely similar isoforms
-
Hordeum vulgare
-
cv. Express
-

Purification (Commentary)

Purification (Comment) Organism
partial purification Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,6-beta-D-fructosyl)n
-
Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + (2,6-beta-D-fructosyl)n transgenic plants Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
?
sucrose + (2,6-beta-D-fructosyl)n biosynthesis of fructans, important in assimilate partitioning and possibly in stress tolerance in plants Hordeum vulgare glucose + (2,6-beta-D-fructosyl)n+1
-
?

Subunits

Subunits Comment Organism
dimer 1 * 49000 + 1 * 23000, SDS-PAGE Hordeum vulgare

Synonyms

Synonyms Comment Organism
6-SFT
-
Hordeum vulgare
sucrose:fructan 6-fructosyltransferase
-
Hordeum vulgare

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
assay at Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.75
-
assay at Hordeum vulgare