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Literature summary for 2.4.1.10 extracted from

  • Biedendieck, R.; Beine, R.; Gamer, M.; Jordan, E.; Buchholz, K.; Seibel, J.; Dijkhuizen, L.; Malten, M.; Jahn, D.
    Export, purification, and activities of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium (2007), Appl. Microbiol. Biotechnol., 74, 1062-1073.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium Limosilactobacillus reuteri

Organism

Organism UniProt Comment Textmining
Limosilactobacillus reuteri
-
-
-
Limosilactobacillus reuteri 21
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of affinity tagged Lactobacillus reuteri levansucrase produced by Bacillus megaterium. The gentle one-step purification method for the recombinant protein directly from the cell-free growth medium provides an alternative to Escherichia coli production and purification systems, where cell disruption, several centrifugation steps, and often more than one purification step are necessary. The one-step purification yields comparable amounts of pure and active enzyme Limosilactobacillus reuteri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru Limosilactobacillus reuteri ?
-
?
alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-galactopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces two galactosyloligofructosides with D-Gal-Fru Limosilactobacillus reuteri 21 ?
-
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside only one mannosyloligofructoside is formed Limosilactobacillus reuteri ?
-
?
alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-mannopyranosyl-1,2-beta-D-fructofuranoside only one mannosyloligofructoside is formed Limosilactobacillus reuteri 21 ?
-
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru Limosilactobacillus reuteri ?
-
?
alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside + alpha-D-xylopyranosyl-1,2-beta-D-fructofuranoside besides the major product, levan, the enzyme produces at least three different xylosyloligofructosides with D-Xyl-Fru Limosilactobacillus reuteri 21 ?
-
?