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Literature summary for 2.4.1.10 extracted from

  • Martinez-Fleites, C.; Ortiz-Lombardia, M.; Pons, T.; Tarbouriech, N.; Taylor, E.J.; Arrieta, J.G.; Hernandez, L.; Davies, G.J.
    Crystal structure of levansucrase from the Gram-negative bacterium Gluconacetobacter diazotrophicus (2005), Biochem. J., 390, 19-27.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Gluconacetobacter diazotrophicus

Crystallization (Commentary)

Crystallization (Comment) Organism
the three-dimensional structure of LsdA determined by X-ray crystallography at a resolution of 2.5 A Gluconacetobacter diazotrophicus

Protein Variants

Protein Variants Comment Organism
C339S kcat/Km for hydrolysis of sucrose is 68.75fold lower than wild-type value Gluconacetobacter diazotrophicus
C395S kcat/Km for hydrolysis of sucrose is 60fold lower than wild-type value Gluconacetobacter diazotrophicus
D135N kcat/Km for hydrolysis of sucrose is 1257fold lower than wild-type value Gluconacetobacter diazotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.9
-
 sucrose 30°C, pH 5.0, wild-type enzyme Gluconacetobacter diazotrophicus
12.3
-
 sucrose 30°C, pH 5.0, mutant enzyme D135N Gluconacetobacter diazotrophicus
13.1
-
 sucrose 30°C, pH 5.0, mutant enzyme C395S Gluconacetobacter diazotrophicus
13.8
-
 sucrose 30°C, pH 5.0, mutant enzyme C339S Gluconacetobacter diazotrophicus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Gluconacetobacter diazotrophicus
-
-

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus Q43998 recombinant enzyme expressed in Escherichia coli
-
Gluconacetobacter diazotrophicus SRT4 Q43998 recombinant enzyme expressed in Escherichia coli
-

Synonyms

Synonyms Comment Organism
LsdA
-
 Gluconacetobacter diazotrophicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0039
-
 sucrose 30°C, pH 5.0, wild-type enzyme Gluconacetobacter diazotrophicus
1.7
-
 sucrose 30°C, pH 5.0, mutant enzyme D135N Gluconacetobacter diazotrophicus
66.9
-
 sucrose 30°C, pH 5.0, mutant enzyme C339S Gluconacetobacter diazotrophicus
71.8
-
 sucrose 30°C, pH 5.0, mutant enzyme C395S Gluconacetobacter diazotrophicus