Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.10 extracted from

  • Esawy, M.; Mahmoud, D.; Fattah, A.
    Immobilisation of Bacillus subtilis NRC33a levansucrase and some studies on its properties (2008), Braz. J. Chem. Eng., 25, 237-246.
No PubMed abstract available
Search for more literature for 2.4.1.10

Protein Variants

Protein Variants Comment Organism
additional information partially purified enzyme immobilization on Ca-alginate beads, or entrapment in agar and agarose, or crosslinking on chitosan by glutaraldehyde, activity of the immobilized enzyme is relatively lower than the activity of the free enzyme. The immobilized levansucrase shows a slight increase in activity compared with the free enzyme above 35°C. The activation energies are 6.62 and 9.27 kcal/mol for free and immobilized enzyme, respectively. The immobilized enzyme shows an increased thermal stability and reduced deactivation energy, as well as increased pH stability at acidic pH Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
AgCl immobilized enzyme: 38% inhibition at 0.1 mM, 67% inhibition at 1 mM, complete inhibition at 10 mM. Free enzyme: 49% inhibition at 0.1 mM, 71% inhibition at 1 mM, 95% inhibition at 10 mM Bacillus subtilis
CuSO4 immobilized enzyme: 25% inhibition at 0.1 mM, 37% inhibition at 1 mM, 65% inhibition at 10 mM. Free enzyme: 30% inhibition at 0.1 mM, 42% inhibition at 1 mM, 79% inhibition at 10 mM Bacillus subtilis
FeCl3 immobilized enzyme: 8% inhibition at 0.1 mM, 21% inhibition at 1 mM, 35% inhibition at 10 mM. Free enzyme: 13% inhibition at 0.1 mM, 29% inhibition at 1 mM, 40% inhibition at 10 mM Bacillus subtilis
HgCl2 immobilized enzyme: 54% inhibition at 0.1 mM, 61% inhibition at 1 mM, 79% inhibition at 10 mM. Free enzyme: 61% inhibition at 0.1 mM, 75% inhibition at 1 mM, 91% inhibition at 10 mM Bacillus subtilis
MgSO4 11% inhibition of the immobilized enzyme at 10 mM, 21% inhibition of the free enzyme at 10 mM Bacillus subtilis
additional information no inhibition by EDTA at 0.1-10 mM Bacillus subtilis
NaCl immobilized enzyme: 20% inhibition at 0.1 mM, 29% inhibition at 1 mM, 50% inhibition at 10 mM. Free enzyme: 26% inhibition at 0.1 mM, 40% inhibition at 1 mM, 55% inhibition at 10 mM Bacillus subtilis
SDS immobilized enzyme: 11% inhibition at 0.1 mM, 21% inhibition at 1 mM, 48% inhibition at 10 mM. Free enzyme: 14% inhibition at 0.1 mM, 27% inhibition at 1 mM, 52% inhibition at 10 mM Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
MnCl2 22% activation of the immobilized enzyme at 0.1 mM, 30% activation at 0.1 mM of the free enzyme Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis NRC33a
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme partially from strain NRC33a Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 free and immobilized enzymes Bacillus subtilis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 50 activity range, free and immobilized enzymes Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 although the thermal stability of the immobilised levansucrase is significantly improved in comparison to the free form, the immobilised enzyme at 50°C loses the activity faster than the free form, inactivation kinetics, overview Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2 6 free and immobilized enzymes Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4 8 activity range, free and immobilized enzymes Bacillus subtilis