Cloned (Comment) | Organism |
---|---|
gene sacB, cloning in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21(DE3), and high xylose-inducible expression of the His6-tagged enzyme in Bacillus megaterium, where the recombinant enzyme is secreted to the medium, method optimization by response-surface methodology, overview. The enhanced secretion of His6-tagged protein is an advantage for purification via the C-terminal His6 tag, which leads to the purification of intact levansucrase, and a 21fold higher compared to the untagged enzyme | Bacillus amyloliquefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, overview | Bacillus amyloliquefaciens | |
47.81 | - |
D-glucose | pH 6.0, 30°C, recombinant enzyme | Bacillus amyloliquefaciens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Bacillus amyloliquefaciens | 5829 | - |
extracellular | secretion of the enzyme | Bacillus amyloliquefaciens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
x * 49000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 sucrose | Bacillus amyloliquefaciens | - |
D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside | - |
? | |
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | Bacillus amyloliquefaciens | - |
D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
type 1, gene sacB | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 4fold by nickel affinity chromatography | Bacillus amyloliquefaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.8 | - |
native enzyme from strain type 1, culture supernatant | Bacillus amyloliquefaciens |
37.32 | - |
purified recombinant His-tagged enzyme from Escherichia coli, release of glucose | Bacillus amyloliquefaciens |
669.6 | - |
recombinant His6-tagged enzyme from Bacillus megaterium, culture supernatant | Bacillus amyloliquefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 sucrose | - |
Bacillus amyloliquefaciens | D-glucose + beta-D-fructofuranosyl-(2,6)-beta-D-fructofuranosyl-(2,1)-alpha-D-glucopyranoside | - |
? | |
sucrose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n alpha-D-glucopyranoside | - |
Bacillus amyloliquefaciens | D-glucose + beta-D-fructofuranosyl-[(2,6)beta-D-fructofuranosyl-]n+1 alpha-D-glucopyranoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 49000, recombinant enzyme expressed in Escherichia coli, SDS-PAGE | Bacillus amyloliquefaciens |
Synonyms | Comment | Organism |
---|---|---|
SacB | - |
Bacillus amyloliquefaciens |
type 1 levansucrase | - |
Bacillus amyloliquefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
levan formation | Bacillus amyloliquefaciens |
30 | - |
D-glucose production | Bacillus amyloliquefaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 60 | - |
Bacillus amyloliquefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
D-glucose production, recombinant enzyme | Bacillus amyloliquefaciens |
8 | - |
levan formation, recombinant enzyme | Bacillus amyloliquefaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | pH activity profile, overview | Bacillus amyloliquefaciens |