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Literature summary for 2.4.1.10 extracted from

  • Li, M.; Seo, S.; Karboune, S.
    Bacillus amyloliquefaciens levansucrase-catalyzed the synthesis of fructooligosaccharides, oligolevan and levan in maple syrup-based reaction systems (2015), Carbohydr. Polym., 133, 203-212.
    View publication on PubMed
Search for more literature for 2.4.1.10

Application

Application Comment Organism
synthesis the enzyme is interesting for levan production due to its ability to directly use the free energy of cleavage of non-activated sucrose to transfer the fructosyl group to a variety of acceptors including monosaccharides (exchange), oligosaccharides (fructooligosaccharides synthesis) or a growing fructan chain (polymer synthesis). Levansucrase from Bacillus amyloliquefaciens is a promising biocatalyst for the synthesis of beta-(2-6)-linked-fructose-based carbohydrates (fructooligosaccharides, oligolevans, levans) targeting specific structures and functional properties Bacillus amyloliquefaciens

Organism

Organism UniProt Comment Textmining
Bacillus amyloliquefaciens P21130 gene sacB
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information levansucrase catalyzes simultaneously both the transfructosylation and the hydrolysis, which is regarded as the transfer of the fructosyl group to water, time courses for transfructosylation and hydrolysis reactions, overview. Measurement of sucrose hydrolysis releasing D-glucose and D-fructose. Disaccharides lactose, cellobiose and melibiose are used as acceptors, with high acceptor specificity towards lactose. Maple syrup is used as assay medium and sucrose source, profile of the end-products synthesized by the enzyme, overview Bacillus amyloliquefaciens ?
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sucrose + cellobiose
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 Bacillus amyloliquefaciens D-glucose + beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside the major transfructosylation product is identified to be cellobiose-fructose (beta-D-glucopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside) ?
sucrose + lactose
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 Bacillus amyloliquefaciens D-glucose + beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside the major transfructosylation product is identified to be lactosefructose or lactosucrose (beta-D-galactopyranosyl-(1->4)-beta-D-glucopyranosyl-(1->2)-beta-D-fructofuranoside) ?
sucrose + melibiose
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 Bacillus amyloliquefaciens D-glucose + raffinose
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
8 30 assays at 8°C and 30°C Bacillus amyloliquefaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
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 assay at Bacillus amyloliquefaciens