Literature summary for 2.4.1.100 extracted from

Van den Ende, W.; Clerens, S.; Vergauwen, R.; Boogaerts, D.; Le Roy, K.; Arckens, L.; Van Laere, A.
Cloning and functional analysis of a high DP fructan:fructan 1-fructosyl transferase from Echinops ritro (Asteraceae): comparison of the native and recombinant enzymes (2006), J. Exp. Bot., 57, 775-789.

Search for more literature for 2.4.1.100

Application

Application	Comment	Organism
agriculture	introduction of the high DP 1-FFT gene in chicory, a crop widely used for inulin extraction, could lead to an increase in degree of polymerization which is useful for a number of specific industrial applications	Echinops ritro
synthesis	the enzyme might be suitable for both fructo-oligosaccharides and high degree of polymerization inulin production in bioreactors	Echinops ritro

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris. The recombinant enzyme is a functional 1-FFT lacking invertase and sucrose:sucrose 1-fructosyl transferase activities, but shows a small intrinsic fructan exohydrolase activity	Echinops ritro

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
19000	-	1 * 19000 + 1 * 52000, SDS-PAGE	Echinops ritro
52000	-	1 * 19000 + 1 * 52000, SDS-PAGE	Echinops ritro

Organism

Organism	UniProt	Comment	Textmining
Echinops ritro	Q0PCC9	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Echinops ritro

Source Tissue

Source Tissue	Comment	Organism	Textmining
root	-	Echinops ritro	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n	-	Echinops ritro	[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1	-	?

Subunits

Subunits	Comment	Organism
dimer	1 * 19000 + 1 * 52000, SDS-PAGE	Echinops ritro

Synonyms

Synonyms	Comment	Organism
1-FFT	-	Echinops ritro
high DP 1-FFT	-	Echinops ritro
high DP fructan:fructan 1-fructosyl transferase	-	Echinops ritro

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)