Literature summary for 2.4.1.100 extracted from

Schroeven, L.; Lammens, W.; Kawakami, A.; Yoshida, M.; Van Laere, A.; Van den Ende, W.
Creating S-type characteristics in the F-type enzyme fructan:fructan 1-fructosyltransferase of Triticum aestivum L (2009), J. Exp. Bot., 60, 3687-3696.

Search for more literature for 2.4.1.100

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Pichia pastoris	Triticum aestivum

Protein Variants

Protein Variants	Comment	Organism
D281Y	the mutation restores the tight H-bond network and introduces typical S-type enzyme characteristics like producing similar amounts of 1-kestotriose compared to sucrose:sucrose 1-fructosyltransferases	Triticum aestivum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
197	-	sucrose	mutant enzyme D281Y, in 50 mM sodium acetate buffer pH 5.0, at 30°C	Triticum aestivum
203	-	sucrose	wild type enzyme, in 50 mM sodium acetate buffer pH 5.0, at 30°C	Triticum aestivum
233	-	1-kestotriose	wild type enzyme, in 50 mM sodium acetate buffer pH 5.0, at 30°C	Triticum aestivum
377	-	1-kestotriose	mutant enzyme D281Y, in 50 mM sodium acetate buffer pH 5.0, at 30°C	Triticum aestivum

Organism

Organism	UniProt	Comment	Textmining
Triticum aestivum	Q4AEI9	-	-

Purification (Commentary)

Purification (Comment)	Organism
Fast Desalting column chromatography	Triticum aestivum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-kestotriose + sucrose	the wild type enzyme clearly prefers 1-kestotriose over sucrose	Triticum aestivum	1,1-nystose	-	?
[beta-D-fructosyl-(2->1)-]m + [beta-D-fructosyl-(2->1)-]n	the enzyme preferentially uses fructan as the donor substrate	Triticum aestivum	[beta-D-fructosyl-(2->1)-]m-1 + [beta-D-fructosyl-(2->1)-]n+1	-	?

Synonyms

Synonyms	Comment	Organism
1-FFT	-	Triticum aestivum
fructan:fructan 1-fructosyltransferase	-	Triticum aestivum

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)