Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Lolium perenne |
Protein Variants | Comment | Organism |
---|---|---|
N340D | the mutant shows only very low overall activity levels | Lolium perenne |
N340D/S415N | the mutant shows similar properties to the W343R mutant, but the 1-nystose/neoketose ratio is decreased | Lolium perenne |
N340D/W343R | the mutant shows 6G-FFT and 1-FFT activities that hold the middle between those of the N340D and W343R single mutants | Lolium perenne |
N340D/W343R/S415N | the triple mutant gains the characteristic of using sucrose as a donor substrate, at the same time, this mutant can still use 1-kestose as a donor substrate, but in a much less efficient way than the wild type | Lolium perenne |
S415N | the mutant shows slightly lower 6G-FFT/1-FFT activities, but the ratio between the main reaction products is not significantly affected | Lolium perenne |
W343R | the mutant shows a wild type1-FFT activity but a largely reduced 6G-FFT activity | Lolium perenne |
General Stability | Organism |
---|---|
several attempts to purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeast's expression medium | Lolium perenne |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
976 | - |
D-sucrose | mutant enzyme N340D/W343R/S415N, in 50 mM sodium acetate buffer, pH 5.0, at 30°C | Lolium perenne |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lolium perenne | Q8S337 | perennial ryegrass | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, several attempts (concanavalin A, Mono Q, Mono S) to further purify 6G-FFT/1-FFT are unsuccessful, probably due to the instability of the enzyme and/or the rather low concentration in the yeasts expression medium | Lolium perenne |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + 1-kestose | 1-FFT activity | Lolium perenne | D-sucrose + 1,1-nystose | - |
? | |
additional information | a minor 1-SST activity is only found with D-sucrose as a single substrate if there is no 1-kestose available, while 1-kestose hydrolytic activity becomes gradually more important at low concentrations | Lolium perenne | ? | - |
? | |
additional information | the bifunctional 6G-FFT/1-FFT uses 1-kestotriose as a preferential donor substrate and can create both a beta(2->1) linkage between two fructosyl residues and a beta(2->6) linkage between one fructosyl residue and one glucosyl residue | Lolium perenne | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
6G-FFT/1-FFT | - |
Lolium perenne |
fructan:fructan 6G-fructosyltransferase/fructan:fructan 1-fructosyltransferase | - |
Lolium perenne |