Protein Variants | Comment | Organism |
---|---|---|
C907T | point mutation in mutant cell line Lec1A.3E and Lec1A.5J, altering interactions important for stabilization of the structure, correction of the mutation by site-directed mutagenesis restores enzyme activity | Cricetulus griseus |
G634A | point mutation in mutant cell line Lec1A.2C, disrution of DxD motif responsible for interaction with UDP-GlcNAc and Mn2+, correction of the mutation by site-directed mutagenesis restores enzyme activity | Cricetulus griseus |
additional information | 1 difference to published sequence with accession number U65791: C1340 is A1340 | Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
UDP-N-acetyl-D-glucosamine | wild-type CHO | Cricetulus griseus | |
0.26 | - |
Man5GlcNAc2 | wild-type CHO | Cricetulus griseus | |
5.43 | - |
Man5GlcNAc2 | mutant Lec1A | Cricetulus griseus | |
7.56 | - |
UDP-N-acetyl-D-glucosamine | mutant Lec1A | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | P70680 | 1 difference to published sequence U65791: C1340 is A1340 | - |
Mesocricetus auratus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] | active site | Cricetulus griseus | |
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] | R303 is important for enzyme activity, conserved throughout all species | Cricetulus griseus | |
UDP-N-acetyl-alpha-D-glucosamine + Man5GlcNAc2-[protein] = UDP + Man5GlcNAc3-[protein] | D212 is the central residue of the DxD motif, conserved throughout all species | Cricetulus griseus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
CHO cell | wild-type, Lec1 cells are deficient in enzyme activity, mutant Lec1A cells show weak enzyme activity, 2% compared to wild-type, and produce complex N-glycans | Cricetulus griseus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
wild-type and mutant cell lines | Cricetulus griseus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + Man5GlcNAc2 | - |
Cricetulus griseus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GlcNAc-T I | - |
Mesocricetus auratus |
GlcNAc-T I | - |
Cricetulus griseus |