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Literature summary for 2.4.1.101 extracted from

  • Nozaki, H.; Matsuzawa, T.; Nakamura, T.; Arai, I.; Urashima, T.
    Are there two forms of beta 2-N-acetylglucosaminyltransferase I in rat testicular and epididymal fluids? (2003), Biochim. Biophys. Acta, 1649, 140-145.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective Rattus norvegicus
Co2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective Rattus norvegicus
Mg2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective Rattus norvegicus
Mn2+ the enzyme in testicular fluid has an absolute requirement for either Co2+, or Mn2+, Mg2+ and ca2+. The activity is stimulated by the cations in the above order. The enzyme from cauda epididymal fluid has an absolute requirement for Mn2+ or Ca2+, with Co2+ and Mg2+ being ineffective Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus the enzyme catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cauda epididymis two forms of beta 2-N-acetylglucosaminyltransferase I in rat testicular and epididymal fluids Rattus norvegicus
-
testis two forms of beta 2-N-acetylglucosaminyltransferase I in rat testicular and cauda epididymal fluids Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins Rattus norvegicus ?
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-3)Man
-
Rattus norvegicus UDP + ?
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-3)Manalpha(1-6)Man
-
Rattus norvegicus UDP + ?
-
?
UDP-N-acetyl-D-glucosamine + Manalpha(1-6)Man
-
Rattus norvegicus UDP + ?
-
?

Synonyms

Synonyms Comment Organism
GnT I
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
enzyme from testicular fluid Rattus norvegicus
7
-
enzyme from epididymal fluid Rattus norvegicus