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Literature summary for 2.4.1.101 extracted from
- Recombinant expression and characterization of N-acetylglucosaminyltransferase I derived from Nicotiana tabacum (2010), J. Biosci. Bioeng., 109, 388-391.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the enzyme fused to maltose-binding protein is expressed in Escherichia coli Rosetta-gami B(DE3) cells | Nicotiana tabacum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.5 | - |
Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | recombinant enzyme, pH not specified in the publication, at 25°C | Nicotiana tabacum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Nicotiana tabacum | 5794 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | maximal activity in the presence of 2 mM Mn2+ | Nicotiana tabacum | |
| additional information | not stimulated by Mg2+, Co2+, Ca2+, Zn2+ and C2+ | Nicotiana tabacum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Nicotiana tabacum | Q9ST97 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography, gel filtration | Nicotiana tabacum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-2)Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | 3.9% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | Nicotiana tabacum | UDP + GlcNAcbeta(1-2)Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
| UDP-N-acetyl-D-glucosamine + Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | 0.5% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | Nicotiana tabacum | UDP + Manalpha(1-2)Manalpha(1-3)[Manalpha(1-6)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
| UDP-N-acetyl-D-glucosamine + Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | 0.7% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | Nicotiana tabacum | UDP + GlcNAcbeta(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
| UDP-N-acetyl-D-glucosamine + Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | 100% activity | Nicotiana tabacum | UDP + Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
| UDP-N-acetyl-D-glucosamine + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | 6.9% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | Nicotiana tabacum | UDP + Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | - |
? | |
| UDP-N-acetyl-D-glucosamine + Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside | 6.2% activity compared to Manalpha(1-6)[Manalpha(1-3)]Manalpha(1-6)[Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside | Nicotiana tabacum | UDP + Manalpha(1-6)[GlcNAcbeta(1-2)Manalpha(1-3)]Manbeta(1-4)GlcNAcbeta(1-4)[Fucalpha(1-3)]GlcNAc-pyridylaminoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GnT I | - |
Nicotiana tabacum |
| N-acetylglucosaminyltransferase I | - |
Nicotiana tabacum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Nicotiana tabacum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
45 | the activity decreases with increasing reaction temperature and is completely abolished at temperatures higher than 45°C. On the other hand, substantial GnTI activity is detected at low temperatures, even at 0°C (approximately 50% of the maximum) | Nicotiana tabacum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | the activity is stable after a 2 h incubation at a temperature lower than 25°C, but it significantly decreases after incubation at 35°C and is completely abolished at temperatures higher than 45°C | Nicotiana tabacum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
- |
Nicotiana tabacum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | activity decreases to less than 15% of the maximum at pH values lower than 5.0 and higher than 8.5 | Nicotiana tabacum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | the enzymatic activity is maintained after a 90-min incubation at pH values 6.0-9.5, but it is mostly abolished at pH values 5.0 and 10.0 | Nicotiana tabacum |
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