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Literature summary for 2.4.1.102 extracted from

  • Toki, D.; Sarkar, M.; Yip, B.; Reck, F.; Joziasse, D.; Fukuda, M.; Schachter, H.; Brockhausen, I.
    Expression of stable human O-glycan core 2 beta-1,6-N-acetylglycosaminyltransferase in Sf9 insect cells (1997), Biochem. J., 325, 63-69.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, truncated enzyme form Homo sapiens

Protein Variants

Protein Variants Comment Organism
T60A site-directed mutagenesis, highly reduced activity Homo sapiens
T60A/T97A site-directed mutagenesis, no activity, no secretion of Sf9 cells Homo sapiens
T97A site-directed mutagenesis, 50% reduced activity Homo sapiens

General Stability

General Stability Organism
freeze-drying and resuspension in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 95% remaining activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tunicamycin inactivation; prevents secretion of recombinant enzyme from Sf9 cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular secretion into medium of the recombinant enzyme from Sf9 cells Homo sapiens
-
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein N-glycans at both glycosylation sites Homo sapiens
glycoprotein binds concanavalin A Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
partially, recombinant from Sf9 insect cells Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, crude enzyme extract, stable up to 1 year Homo sapiens
4°C, stable in solution or after lyophilization for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R
-
Homo sapiens UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R i.e. core class 2 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Homo sapiens