Literature summary for 2.4.1.102 extracted from
Toki, D.; Sarkar, M.; Yip, B.; Reck, F.; Joziasse, D.; Fukuda, M.; Schachter, H.; Brockhausen, I.
Expression of stable human O-glycan core 2 beta-1,6-N-acetylglycosaminyltransferase in Sf9 insect cells (1997), Biochem. J., 325, 63-69.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, truncated enzyme form |
Homo sapiens |
Protein Variants
Protein Variants |
Comment |
Organism |
T60A |
site-directed mutagenesis, highly reduced activity |
Homo sapiens |
T60A/T97A |
site-directed mutagenesis, no activity, no secretion of Sf9 cells |
Homo sapiens |
T97A |
site-directed mutagenesis, 50% reduced activity |
Homo sapiens |
General Stability
General Stability |
Organism |
freeze-drying and resuspension in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 95% remaining activity |
Homo sapiens |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
tunicamycin |
inactivation; prevents secretion of recombinant enzyme from Sf9 cells |
Homo sapiens |
|
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
extracellular |
secretion into medium of the recombinant enzyme from Sf9 cells |
Homo sapiens |
- |
- |
Organism
Organism |
UniProt |
Comment |
Textmining |
Homo sapiens |
- |
- |
- |
Posttranslational Modification
Posttranslational Modification |
Comment |
Organism |
glycoprotein |
N-glycans at both glycosylation sites |
Homo sapiens |
glycoprotein |
binds concanavalin A |
Homo sapiens |
Purification (Commentary)
Purification (Comment) |
Organism |
partially, recombinant from Sf9 insect cells |
Homo sapiens |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
additional information |
- |
- |
Homo sapiens |
Storage Stability
Storage Stability |
Organism |
-70°C, crude enzyme extract, stable up to 1 year |
Homo sapiens |
4°C, stable in solution or after lyophilization for several months |
Homo sapiens |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-N-acetyl-D-galactosaminyl-R |
- |
Homo sapiens |
UDP + beta-D-galactosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R |
i.e. core class 2 |
? |
|
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
37 |
- |
assay at |
Homo sapiens |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
7 |
- |
- |
Homo sapiens |