Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | stimulates up to 20fold | Homo sapiens | |
dithiothreitol | stimulates up to 20fold | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of the soluble catalytic domain in insect cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C100S | mutant enzyme with 10% of wild-type activity | Homo sapiens |
C151S | inactive mutant protein | Homo sapiens |
C172S | inactive mutant protein | Homo sapiens |
C199S | inactive mutant protein | Homo sapiens |
C217S | mutant enzyme with 41% of wild-type activity. Similar Km and Vmax values for Galbeta(1-3)GalNAcalpha-p-nitrophenol, Km-value for UDP-N-acetyl-D-glucosamine is 2.9fold higher than wild-type value. In contrast to wild-type enzyme, the mutant enzyme is not inhibited by sulfhydryl reagents and is not stimulated by reducing agents | Homo sapiens |
C372S | inactive mutant protein | Homo sapiens |
C381S | inactive mutant protein | Homo sapiens |
C413S | inactive mutant protein | Homo sapiens |
C59S | inactive mutant protein | Homo sapiens |
DELTA407-428 | inactive mutant protein | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-2-nitrobenzoic acid | inactivation is partially prevented by prior addition of donor or acceptor substrate and by sulfhydryl reducing agents. 1 mM inhibits 94% | Homo sapiens | |
diethyldicarbonate | 0.2 mM, 74% inhibition | Homo sapiens | |
Hydroxyphenylglyoxal | 19% inhibition at 0.25 mM, complete inhibition at 5-10 mM | Homo sapiens | |
iodoacetate | 0.2 mM inhibits the enzyme 70% | Homo sapiens | |
additional information | enzyme is fully active in presence of 5 mM EDTA | Homo sapiens | |
N-bromosuccinimide | 5 mM, complete inhibition | Homo sapiens | |
NEM | inactivation is partially prevented by prior addition of donor or acceptor substrate and by sulfhydryl reducing agents. 0.2 mM inhibits 42% | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
Galbeta(1-3)GalNAcalpha-p-nitrophenol | pH 7, 37°C, wild-type enzyme | Homo sapiens | |
1.6 | - |
Galbeta(1-3)GalNAcalpha-p-nitrophenol | pH 7, 37°C, mutant enzyme C217S | Homo sapiens | |
1.9 | - |
UDP-N-acetyl-D-glucosamine | pH 7, 37°C, wild-type enzyme | Homo sapiens | |
5.6 | - |
UDP-N-acetyl-D-glucosamine | pH 7, 37°C, mutant enzyme C217S | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ions | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
1 * 58000, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Storage Stability | Organism |
---|---|
-80°C, stable for at least 6 months, wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GalNAcalpha-p-nitrophenol | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
C2GnT1 | - |
Homo sapiens |
core 2 beta6-N-acetylglucosaminyltransferase | - |
Homo sapiens |