Cloned (Comment) | Organism |
---|---|
chromosome mapping of PMT1 | Saccharomyces cerevisiae |
DNA sequence determination, chromosome mapping | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | PMT2 disruption mutant shows reduced in vitro and in vivo activity | Saccharomyces cerevisiae |
additional information | PMT1 and PMT2 double disruption mutant shows severe growth defect but retain residual activity due to an additional enzyme | Saccharomyces cerevisiae |
additional information | PMT2 haploid mutants grow slightly slower than the wild-type, the enzyme is required but not essential for normal vegetative growth of the cells | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | integral, multiple transmembranal domains | Saccharomyces cerevisiae | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + protein | Saccharomyces cerevisiae | - |
dolichyl phosphate + O-D-mannosylprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
gene PMT1 | - |
Saccharomyces cerevisiae | - |
enzyme form other than PMT2 | - |
Saccharomyces cerevisiae | P31382 | PMT2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | PMT1 and PMT2 contain both 3 putative N-glycosylation sites | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dolichyl phosphate D-mannose + Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosyl-Ac-Tyr-Asn-Pro-Thr-Ser-Val-NH2 | - |
? | |
dolichyl phosphate D-mannose + protein | - |
Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? | |
dolichyl phosphate D-mannose + protein | the enzyme transfers mannosyl residues to the hydroxyl of serine or threonine residues | Saccharomyces cerevisiae | dolichyl phosphate + O-D-mannosylprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PMT | - |
Saccharomyces cerevisiae |