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Literature summary for 2.4.1.111 extracted from

  • Ibrahim, R.K.; Grisebach, H.
    Purification and properties of UDP-glucose: coniferyl alcohol glucosyltransferase from suspension cultures of Pauls scarlet rose (1976), Arch. Biochem. Biophys., 176, 700-708.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
coniferyl alcohol above 0.08 mM, Paul's scarlet rose Rosa sp.
EDTA 1 mM, 25% inhibition Cicer arietinum
EDTA 1 mM, 25% inhibition Glycine max
EDTA 1 mM, 25% inhibition Linum usitatissimum
EDTA 1 mM, 25% inhibition Nicotiana tabacum
EDTA 1 mM, 25% inhibition Parthenocissus tricuspidata
EDTA 1 mM, 25% inhibition Petroselinum crispum
EDTA 1 mM, 25% inhibition Rosa sp.
PCMB 0.1 mM, 70% inhibition Rosa sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
coniferyl alcohol
-
Rosa sp.
0.0056
-
sinapyl alcohol
-
Rosa sp.
0.02
-
UDPglucose
-
Rosa sp.
0.065
-
coniferyl aldehyde
-
Rosa sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
gel filtration Rosa sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDPglucose + coniferyl alcohol Nicotiana tabacum the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis ?
-
?
UDPglucose + coniferyl alcohol Glycine max the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis ?
-
?
UDPglucose + coniferyl alcohol Petroselinum crispum the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis ?
-
?
UDPglucose + coniferyl alcohol Cicer arietinum the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis ?
-
?
UDPglucose + coniferyl alcohol Linum usitatissimum the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis ?
-
?
UDPglucose + coniferyl alcohol Rosa sp. the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis ?
-
?
UDPglucose + coniferyl alcohol Parthenocissus tricuspidata the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Cicer arietinum
-
weak activity
-
Glycine max
-
v. Kanrich
-
Linum usitatissimum
-
-
-
Nicotiana tabacum
-
weak activity
-
Parthenocissus tricuspidata
-
weak activity
-
Petroselinum crispum
-
weak activity
-
Rosa sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rosa sp.

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
Nicotiana tabacum
-
callus
-
Glycine max
-
callus
-
Linum usitatissimum
-
callus
-
Rosa sp.
-
callus
-
Parthenocissus tricuspidata
-
cell suspension culture
-
Glycine max
-
cell suspension culture
-
Petroselinum crispum
-
cell suspension culture
-
Cicer arietinum
-
cell suspension culture
-
Linum usitatissimum
-
cell suspension culture
-
Parthenocissus tricuspidata
-
cell suspension culture 10-day-old, dark-grown suspension culture Rosa sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.036
-
Paul's scarlet rose Rosa sp.

Storage Stability

Storage Stability Organism
-20°C, addition of 28 mM 2-mercaptoethanol and 10% ethyleneglycol are required for stability, about 20% loss of activity within several weeks Rosa sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + quercetin glucosylated at 22% of the activity with coniferyl alcohol Rosa sp. UDP + quercetin 7-O-beta-D-glucoside
-
?
UDP-glucose + caffeic acid glucosylated at 18% of the activity with coniferyl alcohol Rosa sp. UDP + ?
-
?
UDP-glucose + coniferyl alcohol
-
Nicotiana tabacum UDP + coniferin i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside ?
UDP-glucose + coniferyl alcohol
-
Glycine max UDP + coniferin i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside ?
UDP-glucose + coniferyl alcohol
-
Petroselinum crispum UDP + coniferin i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside ?
UDP-glucose + coniferyl alcohol
-
Cicer arietinum UDP + coniferin i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside ?
UDP-glucose + coniferyl alcohol
-
Linum usitatissimum UDP + coniferin i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside ?
UDP-glucose + coniferyl alcohol
-
Rosa sp. UDP + coniferin i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside ?
UDP-glucose + coniferyl alcohol
-
Parthenocissus tricuspidata UDP + coniferin i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside ?
UDP-glucose + coniferyl aldehyde glucosylated at 46% of the activity with coniferyl alcohol Rosa sp. UDP + coniferaldehyde-4-O-beta-D-glucopyranoside
-
?
UDP-glucose + ferulic acid glucosylated at 20% of the activitry with coniferyl alcohol Rosa sp. UDP + ferulic acid-4-O-beta-D-glucopyranoside
-
?
UDP-glucose + isorhamnetin glucosylated at 22% of the activity with coniferyl alcohol Rosa sp. UDP + isorhamnetin 7-O-beta-D-glucopyranoside
-
?
UDP-glucose + scopoletin
-
Rosa sp. UDP + scopoletin 7-O-beta-D-glucopyranoside
-
?
UDP-glucose + sinapic acid glucosylated at 21% of the activity with coniferyl alcohol Rosa sp. UDP + ?
-
?
UDP-glucose + sinapyl alcohol
-
Glycine max UDP + syringin
-
?
UDP-glucose + sinapyl alcohol glucosylated at 74% of the activity with coniferyl alcohol Rosa sp. UDP + syringin
-
?
UDPglucose + coniferyl alcohol the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis Nicotiana tabacum ?
-
?
UDPglucose + coniferyl alcohol the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis Glycine max ?
-
?
UDPglucose + coniferyl alcohol the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis Petroselinum crispum ?
-
?
UDPglucose + coniferyl alcohol the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis Cicer arietinum ?
-
?
UDPglucose + coniferyl alcohol the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis Linum usitatissimum ?
-
?
UDPglucose + coniferyl alcohol the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis Rosa sp. ?
-
?
UDPglucose + coniferyl alcohol the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis Parthenocissus tricuspidata ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
Tris-HCl buffer Rosa sp.
8
-
phosphate buffer Rosa sp.

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.4 about half-maximal activity at pH 6.5 and 8.4, Tris buffer Rosa sp.
7 9.2 about half-maximal activity at pH 7 and 9.2, phosphate buffer Rosa sp.