Inhibitors | Comment | Organism | Structure |
---|---|---|---|
coniferyl alcohol | above 0.08 mM, Paul's scarlet rose | Rosa sp. | |
EDTA | 1 mM, 25% inhibition | Cicer arietinum | |
EDTA | 1 mM, 25% inhibition | Glycine max | |
EDTA | 1 mM, 25% inhibition | Linum usitatissimum | |
EDTA | 1 mM, 25% inhibition | Nicotiana tabacum | |
EDTA | 1 mM, 25% inhibition | Parthenocissus tricuspidata | |
EDTA | 1 mM, 25% inhibition | Petroselinum crispum | |
EDTA | 1 mM, 25% inhibition | Rosa sp. | |
PCMB | 0.1 mM, 70% inhibition | Rosa sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
coniferyl alcohol | - |
Rosa sp. | |
0.0056 | - |
sinapyl alcohol | - |
Rosa sp. | |
0.02 | - |
UDPglucose | - |
Rosa sp. | |
0.065 | - |
coniferyl aldehyde | - |
Rosa sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Rosa sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + coniferyl alcohol | Nicotiana tabacum | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | ? | - |
? | |
UDPglucose + coniferyl alcohol | Glycine max | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | ? | - |
? | |
UDPglucose + coniferyl alcohol | Petroselinum crispum | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | ? | - |
? | |
UDPglucose + coniferyl alcohol | Cicer arietinum | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | ? | - |
? | |
UDPglucose + coniferyl alcohol | Linum usitatissimum | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | ? | - |
? | |
UDPglucose + coniferyl alcohol | Rosa sp. | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | ? | - |
? | |
UDPglucose + coniferyl alcohol | Parthenocissus tricuspidata | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cicer arietinum | - |
weak activity | - |
Glycine max | - |
v. Kanrich | - |
Linum usitatissimum | - |
- |
- |
Nicotiana tabacum | - |
weak activity | - |
Parthenocissus tricuspidata | - |
weak activity | - |
Petroselinum crispum | - |
weak activity | - |
Rosa sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rosa sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
callus | - |
Nicotiana tabacum | - |
callus | - |
Glycine max | - |
callus | - |
Linum usitatissimum | - |
callus | - |
Rosa sp. | - |
callus | - |
Parthenocissus tricuspidata | - |
cell suspension culture | - |
Glycine max | - |
cell suspension culture | - |
Petroselinum crispum | - |
cell suspension culture | - |
Cicer arietinum | - |
cell suspension culture | - |
Linum usitatissimum | - |
cell suspension culture | - |
Parthenocissus tricuspidata | - |
cell suspension culture | 10-day-old, dark-grown suspension culture | Rosa sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.036 | - |
Paul's scarlet rose | Rosa sp. |
Storage Stability | Organism |
---|---|
-20°C, addition of 28 mM 2-mercaptoethanol and 10% ethyleneglycol are required for stability, about 20% loss of activity within several weeks | Rosa sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + quercetin | glucosylated at 22% of the activity with coniferyl alcohol | Rosa sp. | UDP + quercetin 7-O-beta-D-glucoside | - |
? | |
UDP-glucose + caffeic acid | glucosylated at 18% of the activity with coniferyl alcohol | Rosa sp. | UDP + ? | - |
? | |
UDP-glucose + coniferyl alcohol | - |
Nicotiana tabacum | UDP + coniferin | i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside | ? | |
UDP-glucose + coniferyl alcohol | - |
Glycine max | UDP + coniferin | i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside | ? | |
UDP-glucose + coniferyl alcohol | - |
Petroselinum crispum | UDP + coniferin | i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside | ? | |
UDP-glucose + coniferyl alcohol | - |
Cicer arietinum | UDP + coniferin | i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside | ? | |
UDP-glucose + coniferyl alcohol | - |
Linum usitatissimum | UDP + coniferin | i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside | ? | |
UDP-glucose + coniferyl alcohol | - |
Rosa sp. | UDP + coniferin | i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside | ? | |
UDP-glucose + coniferyl alcohol | - |
Parthenocissus tricuspidata | UDP + coniferin | i.e. coniferyl alcohol 7-O-beta-D-glucopyranoside | ? | |
UDP-glucose + coniferyl aldehyde | glucosylated at 46% of the activity with coniferyl alcohol | Rosa sp. | UDP + coniferaldehyde-4-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + ferulic acid | glucosylated at 20% of the activitry with coniferyl alcohol | Rosa sp. | UDP + ferulic acid-4-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + isorhamnetin | glucosylated at 22% of the activity with coniferyl alcohol | Rosa sp. | UDP + isorhamnetin 7-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + scopoletin | - |
Rosa sp. | UDP + scopoletin 7-O-beta-D-glucopyranoside | - |
? | |
UDP-glucose + sinapic acid | glucosylated at 21% of the activity with coniferyl alcohol | Rosa sp. | UDP + ? | - |
? | |
UDP-glucose + sinapyl alcohol | - |
Glycine max | UDP + syringin | - |
? | |
UDP-glucose + sinapyl alcohol | glucosylated at 74% of the activity with coniferyl alcohol | Rosa sp. | UDP + syringin | - |
? | |
UDPglucose + coniferyl alcohol | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | Nicotiana tabacum | ? | - |
? | |
UDPglucose + coniferyl alcohol | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | Glycine max | ? | - |
? | |
UDPglucose + coniferyl alcohol | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | Petroselinum crispum | ? | - |
? | |
UDPglucose + coniferyl alcohol | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | Cicer arietinum | ? | - |
? | |
UDPglucose + coniferyl alcohol | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | Linum usitatissimum | ? | - |
? | |
UDPglucose + coniferyl alcohol | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | Rosa sp. | ? | - |
? | |
UDPglucose + coniferyl alcohol | the role of this enzyme is believed to catalyze the transfer of glucose from UDPglucose to coniferyl or sinapyl alcohol as storage intermediates in lignin biosynthesis | Parthenocissus tricuspidata | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
Tris-HCl buffer | Rosa sp. |
8 | - |
phosphate buffer | Rosa sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.4 | about half-maximal activity at pH 6.5 and 8.4, Tris buffer | Rosa sp. |
7 | 9.2 | about half-maximal activity at pH 7 and 9.2, phosphate buffer | Rosa sp. |