Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | detergent required for maximal activity | Silene dioica | |
2-Methoxyethanol | activation | Silene dioica | |
cetrimide | activation | Silene dioica | |
cysteine | detergent required for maximal activity | Silene dioica | |
Triton X-100 | activation | Silene dioica | |
Tween 20 | detergent required for maximal activity | Silene dioica | |
Tween 80 | detergent required for maximal activity | Silene dioica |
General Stability | Organism |
---|---|
repeated freeze-thawing inactivates | Silene dioica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 2 mM, 18% inhibition | Silene dioica | |
HgCl2 | 0.1 mM, 85% inhibition | Silene dioica | |
NEM | 1.25 mM, 30% inhibition | Silene dioica | |
PCMB | 1.25 mM, 93% inhhibition, 2-mercaptoethanol or cysteine restores activity | Silene dioica | |
ZnCl2 | 2 mM, 22% inhibition | Silene dioica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
cyanidin | - |
Silene dioica | |
0.13 | - |
delphinidin | - |
Silene dioica | |
0.13 | - |
pelargonidin | - |
Silene dioica | |
0.41 | - |
UDPglucose | - |
Silene dioica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
1 * 60000, can exist as monomer or dimer, SDS-PAGE | Silene dioica |
60000 | - |
2 * 60000, can exist as monomer or dimer, SDS-PAGE | Silene dioica |
125000 | - |
gel filtration | Silene dioica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + anthocyanidin | Silene dioica | involved in anthocyanine biosynthesis | UDP + anthocyanidin-3-O-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Silene dioica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Silene dioica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
calyx | - |
Silene dioica | - |
leaf | young rosette leaves | Silene dioica | - |
petal | highest activity in petals of opening flowers of young plants | Silene dioica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0252 | - |
- |
Silene dioica |
Storage Stability | Organism |
---|---|
-17°C, about 40% loss of activity within 3 months | Silene dioica |
4°C, about 40% loss of activity within 1 week | Silene dioica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + delphinidin | more slowly than cyanidin | Silene dioica | UDP + delphinidin 3-O-glucoside | - |
? | |
UDP-glucose + pelargonidin | more slowly than cyanidin | Silene dioica | UDP + pelargonidin 3-O-glucoside | - |
? | |
UDPglucose + anthocyanidin | involved in anthocyanine biosynthesis | Silene dioica | UDP + anthocyanidin-3-O-glucoside | - |
? | |
UDPglucose + cyanidin | best substrate | Silene dioica | UDP + cyanidin 3-O-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 60000, can exist as monomer or dimer, SDS-PAGE | Silene dioica |
monomer | 1 * 60000, can exist as monomer or dimer, SDS-PAGE | Silene dioica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Silene dioica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.6 | 8 | 50% of maximal activity at pH 6.6 and pH 8.0 | Silene dioica |