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Literature summary for 2.4.1.115 extracted from
- Functional characterization of a UDP-glucose:flavonoid 3-O-beta-D-glucosyltransferase from the seed coat of black soybean (Glycine max (L.) Merr.) (2010), Phytochemistry, 71, 1253-1263.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in the Arabidopsis thaliana T-DNA mutant (ugt78d2) deficient in anthocyanidin and flavonol 3-O-beta-D-glucosyltransferase activity and in Escherichia coli BL21(DE3) pLysS cells | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.174 | - |
kaempferol | recombinant enzyme, at pH 8.0 and 30°C | Glycine max |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 49240 | - |
x * 49240, calculated from amino acid sequence | Glycine max |
| 49800 | - |
x * 49800, SDS-PAGE | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | D3Y5N8 | cultivar Clark line | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Talon metal affinity column chromatography, gel filtration | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| embryo | - |
Glycine max | - |
| flower bud | - |
Glycine max | - |
| leaf | - |
Glycine max | - |
| pod | - |
Glycine max | - |
| root | - |
Glycine max | - |
| seed coat | - |
Glycine max | - |
| stem | - |
Glycine max | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, 50% glycerol solution, 24 h, no significant loss of activity | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity is observed for dihydroflavonol (dihydroquercetin), isoflavone (genistin, genistein and daidzein), (epi)-flavan-3-O-beta-Dl (catechin, epicatechin), 3-deoxyanthocyanidin (apigeninidin), flavonol conjugate (flavonol 3-O-beta-D-glucosides) and anthocyanins (anthocyanidin 3-O-beta-D-glucosides and cyanidin 3-O-beta-D-galactoside). Galactose can be transferred from UDP-galactose to the 3-position of cyanidin and delphinidin with low specificity relative to the analogous glucosyltransferase reactions | Glycine max | ? | - |
? | |
| UDP-glucose + cyanidin | - |
Glycine max | UDP + cyanidin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + delphinidin | when acceptor substrates are supplied below the inhibitory concentration of cyaniding, the enzyme demonstrates highest activity towards the anthocyanidin delphinidin | Glycine max | UDP + delphinidin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + isorhamnetin | - |
Glycine max | UDP + isorhamnetin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + kaempferol | when acceptor substrates are supplied above the inhibitory concentration of cyanidin, the enzyme demonstrates highest activity for flavonols (quercetin, myricetin, and kaempferol) | Glycine max | UDP + kaempferol-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + malvidin | - |
Glycine max | UDP + malvidin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + myricetin | when acceptor substrates are supplied above the inhibitory concentration of cyanidin , the enzyme demonstrates highest activity for flavonols (quercetin, myricetin, and kaempferol) | Glycine max | UDP + myricetin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + pelargonidin | - |
Glycine max | UDP + pelargonidin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + peonidin | - |
Glycine max | UDP + peonidin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + petunidin | - |
Glycine max | UDP + petunidin-3-O-beta-D-glucoside | - |
? | |
| UDP-glucose + quercetin | when acceptor substrates are supplied above the inhibitory concentration of cyanidin , the enzyme demonstrates highest activity for flavonols (quercetin, myricetin, and kaempferol) | Glycine max | UDP + quercetin-3-O-beta-D-glucoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49800, SDS-PAGE | Glycine max |
| ? | x * 49240, calculated from amino acid sequence | Glycine max |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose:flavonoid 3-O-glucosyltransferase | - |
Glycine max |
| UF3GT | - |
Glycine max |
| UGT78K1 | - |
Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Glycine max |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.25 | - |
kaempferol | recombinant enzyme, at pH 8.0 and 30°C | Glycine max | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
the recombinant enzyme exhibits a slight buffer preference for HEPES over Tris buffer at pH 8.0 | Glycine max |
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