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Literature summary for 2.4.1.115 extracted from

  • Hall, D.; Yuan, X.X.; Murata, J.; De Luca, V.
    Molecular cloning and biochemical characterization of the UDP-glucose: flavonoid 3-O-beta-D-glucosyltransferase from Concord grape (Vitis labrusca) (2012), Phytochemistry, 74, 90-99.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DE3 pLysS cells Vitis labrusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00216
-
quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
0.00479
-
cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
0.914
-
UDP-glucose in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50100
-
x * 50100, calculated from amino acid sequence Vitis labrusca

Organism

Organism UniProt Comment Textmining
Vitis labrusca A6N928 cultivar Concord
-

Purification (Commentary)

Purification (Comment) Organism
glutathione Sepharose column chromatography Vitis labrusca

Source Tissue

Source Tissue Comment Organism Textmining
exocarp pre-veraison exocarp Vitis labrusca
-
flower
-
Vitis labrusca
-
flower bud
-
Vitis labrusca
-
leaf
-
Vitis labrusca
-
mesocarp
-
Vitis labrusca
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is not able to further glucosylate cyanidin 3-O-beta-D-glucoside and it could not use UDP-galactose as a donor substrate Vitis labrusca ?
-
?
UDP-glucose + (2R,3R)-dihydroquercetin worst substrate Vitis labrusca UDP + (2R,3R)-dihydroquercetin 3-O-beta-D-glucoside
-
?
UDP-glucose + cyanidin
-
Vitis labrusca UDP + cyanidin-3-O-beta-D-glucoside
-
?
UDP-glucose + delphinidin
-
Vitis labrusca UDP + delphinidin-3-O-beta-D-glucoside
-
?
UDP-glucose + isorhamnetin
-
Vitis labrusca UDP + isorhamnetin-3-O-beta-D-glucoside
-
?
UDP-glucose + kaempferol worst substrate Vitis labrusca UDP + kaempferol-3-O-beta-D-glucoside
-
?
UDP-glucose + malvidin best substrate Vitis labrusca UDP + malvidin-3-O-beta-D-glucoside
-
?
UDP-glucose + peonidin
-
Vitis labrusca UDP + peonidin-3-O-beta-D-glucoside
-
?
UDP-glucose + quercetin while the recombinant enzyme preferentially glucosylates the 3 position of quercetin it is also able to glucosylate position 7 to a small extent in the early stages of the time course Vitis labrusca UDP + quercetin-3-O-beta-D-glucoside
-
?

Subunits

Subunits Comment Organism
? x * 50100, calculated from amino acid sequence Vitis labrusca

Synonyms

Synonyms Comment Organism
3GT
-
Vitis labrusca
L3GT
-
Vitis labrusca
UDP-glucose: flavonoid 3-O-glucosyltransferase
-
Vitis labrusca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000113
-
quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
0.000247
-
UDP-glucose in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
0.000704
-
cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca

Expression

Organism Comment Expression
Vitis labrusca gene expression is highest 12 weeks after flowering in the exocarp up

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.00027
-
UDP-glucose in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
0.052
-
quercetin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca
0.146
-
cyanidin in 100 mM Tris-HCl pH 8.0, at 30°C Vitis labrusca