Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DE3 pLysS cells | Vitis labrusca |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00216 | - |
quercetin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
0.00479 | - |
cyanidin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
0.914 | - |
UDP-glucose | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50100 | - |
x * 50100, calculated from amino acid sequence | Vitis labrusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis labrusca | A6N928 | cultivar Concord | - |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Vitis labrusca |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
exocarp | pre-veraison exocarp | Vitis labrusca | - |
flower | - |
Vitis labrusca | - |
flower bud | - |
Vitis labrusca | - |
leaf | - |
Vitis labrusca | - |
mesocarp | - |
Vitis labrusca | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is not able to further glucosylate cyanidin 3-O-beta-D-glucoside and it could not use UDP-galactose as a donor substrate | Vitis labrusca | ? | - |
? | |
UDP-glucose + (2R,3R)-dihydroquercetin | worst substrate | Vitis labrusca | UDP + (2R,3R)-dihydroquercetin 3-O-beta-D-glucoside | - |
? | |
UDP-glucose + cyanidin | - |
Vitis labrusca | UDP + cyanidin-3-O-beta-D-glucoside | - |
? | |
UDP-glucose + delphinidin | - |
Vitis labrusca | UDP + delphinidin-3-O-beta-D-glucoside | - |
? | |
UDP-glucose + isorhamnetin | - |
Vitis labrusca | UDP + isorhamnetin-3-O-beta-D-glucoside | - |
? | |
UDP-glucose + kaempferol | worst substrate | Vitis labrusca | UDP + kaempferol-3-O-beta-D-glucoside | - |
? | |
UDP-glucose + malvidin | best substrate | Vitis labrusca | UDP + malvidin-3-O-beta-D-glucoside | - |
? | |
UDP-glucose + peonidin | - |
Vitis labrusca | UDP + peonidin-3-O-beta-D-glucoside | - |
? | |
UDP-glucose + quercetin | while the recombinant enzyme preferentially glucosylates the 3 position of quercetin it is also able to glucosylate position 7 to a small extent in the early stages of the time course | Vitis labrusca | UDP + quercetin-3-O-beta-D-glucoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50100, calculated from amino acid sequence | Vitis labrusca |
Synonyms | Comment | Organism |
---|---|---|
3GT | - |
Vitis labrusca |
L3GT | - |
Vitis labrusca |
UDP-glucose: flavonoid 3-O-glucosyltransferase | - |
Vitis labrusca |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000113 | - |
quercetin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
0.000247 | - |
UDP-glucose | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
0.000704 | - |
cyanidin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca |
Organism | Comment | Expression |
---|---|---|
Vitis labrusca | gene expression is highest 12 weeks after flowering in the exocarp | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00027 | - |
UDP-glucose | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
0.052 | - |
quercetin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
0.146 | - |
cyanidin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca |