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Literature summary for 2.4.1.115 extracted from

  • Sui, X.; Gao, X.; Ao, M.; Wang, Q.; Yang, D.; Wang, M.; Fu, Y.; Wang, L.
    cDNA cloning and characterization of UDP-glucose: anthocyanidin 3-O-glucosyltransferase in Freesia hybrida (2011), Plant Cell Rep., 30, 1209-1218.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21 (DE3) cells and in Petunia grandiflora by Agrobacterium-mediated transformation Freesia hybrid cultivar

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48043
-
x * 48043, calculated from amino acid sequence Freesia hybrid cultivar

Organism

Organism UniProt Comment Textmining
Freesia hybrid cultivar E2IQH7
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography Freesia hybrid cultivar

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Freesia hybrid cultivar
-
petal expression is higher in all of the pigmented petals than in stems and leaves Freesia hybrid cultivar
-
stem
-
Freesia hybrid cultivar
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + cyanidin
-
Freesia hybrid cultivar UDP + cyanidin-3-O-beta-D-glucoside
-
?

Subunits

Subunits Comment Organism
? x * 48043, calculated from amino acid sequence Freesia hybrid cultivar

Synonyms

Synonyms Comment Organism
3GT1
-
Freesia hybrid cultivar
UDP-glucose: anthocyanidin 3-O-glucosyltransferase
-
Freesia hybrid cultivar
UF3GT
-
Freesia hybrid cultivar

pI Value

Organism Comment pI Value Maximum pI Value
Freesia hybrid cultivar calculated from amino acid sequence
-
6

Expression

Organism Comment Expression
Freesia hybrid cultivar transcript level in the unpigmented buds is low, but increases during the pigment-accumulation stages and peaks in fully opened petals. Enzyme expression level is identical in red and pink petals and is lower in yellow petals, and rarely detectable in white petals up

General Information

General Information Comment Organism
metabolism the enzyme has a role in anthocyanin glycoside biosynthesis in vitro Freesia hybrid cultivar