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Literature summary for 2.4.1.118 extracted from
- Preparation and characterization, using high-performance liquid chromatography, of an enzyme forming glucosides of cytokinins (1979), Biochim. Biophys. Acta, 570, 124-139.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.15 | - |
zeatin | - |
Raphanus sativus |  |
| 0.19 | - |
UDPglucose | - |
Raphanus sativus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46500 | - |
gel filtration | Raphanus sativus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDPglucose + N6-alkylaminopurine | Raphanus sativus | involved in regulation of cytokinin activity | UDP + N6-alkylaminopurine-7-beta-D-glucoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Raphanus sativus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Raphanus sativus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Raphanus sativus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00046 | - |
- |
Raphanus sativus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| TDP-glucose + zeatin | as effective as UDPglucose | Raphanus sativus | TDP + zeatin 7-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + trans-zeatin | i.e. N6-(4-hydroxy-3-methylbut-trans-2-enylamino)purine | Raphanus sativus | UDP + trans-zeatin 7-beta-D-glucoside | + zeatin 9-glucoside | ? | |
| UDPglucose + 6-benzylaminopurine | activity is 15% higher for 6-benzylaminopurine than for zeatin | Raphanus sativus | UDP + 6-benzylaminopurine-7-beta-D-glucoside | - |
? | |
| UDPglucose + dihydroxyzeatin | - |
Raphanus sativus | UDP + dihydroxyzeatin 7-beta-D-glucoside | - |
? | |
| UDPglucose + N6-alkylaminopurine | - |
Raphanus sativus | UDP + N6-alkylaminopurine-7-beta-D-glucoside | - |
? | |
| UDPglucose + N6-alkylaminopurine | involved in regulation of cytokinin activity | Raphanus sativus | UDP + N6-alkylaminopurine-7-beta-D-glucoside | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | 7.4 | - |
Raphanus sativus |
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