Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | cold-inducible GolS-1 and 2 genes | Ajuga reptans |
Cloned (Comment) | Organism |
---|---|
cold-inducible GolS-1 and -2 genes encode 2 distinct galactinol synthases, cloning and sequencing of the GolS-1 and -2 genes, deduced amino acid sequences, expression of GolS-1 cDNA in Escherichia coli as functional enzyme | Ajuga reptans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + myo-inositol | Ajuga reptans | galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction | UDP + 1-O-alpha-D-galactosyl-D-myo-inositol | - |
? | |
UDP-galactose + myo-inositol | Ajuga reptans | first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance | UDP + 1-O-alpha-D-galactosyl-D-myo-inositol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ajuga reptans | Q9XGN3 | GolS-2, 2 isoforms: GolS-1 and GolS-2 | - |
Ajuga reptans | Q9XGN4 | GolS-1, 2 isoforms: GolS-1 and GolS-2 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | putative serine phosphorylation site at GolS-1 codon position 263 | Ajuga reptans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | source and sink leaves, GolS-1 is source leaf-specific, equally weak expression of GolS-2 in all leaf types | Ajuga reptans | - |
mesophyll | GolS-1 is primarily expressed in the mesophyll, the site of raffinose family oligosaccharides storage | Ajuga reptans | - |
phloem | GolS-2 is primarily expressed in the phloem-associated intermediary cells known for their role in raffinose family oligosaccharides phloem loading | Ajuga reptans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + myo-inositol | reaction is probably reversible | Ajuga reptans | UDP + 1-O-alpha-D-galactosyl-D-myo-inositol | identical with galactinol | r | |
UDP-galactose + myo-inositol | galactinol synthesis, which is the galactosyl donor for the synthesis of raffinose and stachyose, at least 2 isoforms: GolS-1 is mainly involved in the synthesis of storage raffinose family oligosaccharides and GolS-2 in the synthesis of transport raffinose family oligosaccharides, GolS-2 expression is much lower than that of GolS-1, galactinol may be catabolized by the reverse reaction | Ajuga reptans | UDP + 1-O-alpha-D-galactosyl-D-myo-inositol | - |
? | |
UDP-galactose + myo-inositol | first committed enzyme in biosynthesis of raffinose family oligosaccharides, which play a role in plant stress tolerance | Ajuga reptans | UDP + 1-O-alpha-D-galactosyl-D-myo-inositol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Ajuga reptans |