Cloned (Comment) | Organism |
---|---|
gene MfGolS1, cloned from the cold-treated leaves by reverse transcription PCR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time quantitative PCR enzyme expression analysis, recombinant overexpression in Nicotiana tabacum cv. Zhongyan 90 seeds. Overexpression of MfGolS1 in tobacco results in elevated tolerance to freezing and chilling in transgenic plants as a result of enhanced levels of galactinol, raffinose and stachyose. Tolerance to drought and salt stresses is also increased in the transgenic tobacco plants | Medicago falcata |
gene MsGolS1, sequence comparisons and phylogenetic analysis, quantitative real-time quantitative PCR enzyme expression analysis | Medicago sativa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago falcata | B9VV96 | i.e. M. falcata, cv. Hulunbeir, gene MfGolS1 | - |
Medicago sativa | - |
cv. WL-525HQ, gene MsGolS1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Medicago sativa | - |
leaf | - |
Medicago falcata | - |
additional information | gene MfGolS1 transcript is not detected in untreated vegetative tissues, but greatly induced in leaves and slightly in lateral roots, but not in axial roots, stem and petioles, after cold treatment | Medicago falcata | - |
root | lateral | Medicago falcata | - |
Synonyms | Comment | Organism |
---|---|---|
galactinol synthase | - |
Medicago sativa |
galactinol synthase | - |
Medicago falcata |
GAS | - |
Medicago sativa |
GAS | - |
Medicago falcata |
GOLS | - |
Medicago sativa |
GOLS | - |
Medicago falcata |
Organism | Comment | Expression |
---|---|---|
Medicago falcata | the enzyme is greatly induced in leaves, but not in stem and petiole, after cold treatment. MfGolS1 can be induced by myo-inositol, which is proposed to participate in cold-induced MfGolS1 expression. MfGolS1 transcript is weakly induced by dehydration and salt stresses, but not responsive to abscisic acid | up |
Medicago sativa | the enzyme is slightly and short-time induced by cold treatment with low levels of accumulation of sugars including sucrose, galactinol, raffinose, and stachyose | up |
General Information | Comment | Organism |
---|---|---|
physiological function | galactinol synthase catalyzes formation of galactinol and is the key enzyme in biosynthesis of raffinose family oligosaccharides | Medicago sativa |
physiological function | galactinol synthase catalyzes formation of galactinol and is the key enzyme in biosynthesis of raffinose family oligosaccharides. Enzyme MfGolS1 plays an important role in cold tolerance of Medicago falcata. Myoinositol may participate in cold-induced transcription of MfGolS1 that confers multiple tolerances to abiotic stresses. During cold acclimation, sugars including sucrose, galactinol, raffinose, and stachyose are accumulated. The enzyme plays an important role in plant tolerance to abiotic stresses | Medicago falcata |