General Stability | Organism |
---|---|
beta-mercaptoethanol, 10 mM, stabilizes, 80% restorage of activity by its addition after treatment with p-chloromercuribenzoate | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 0.1-10 mM: 20-50% inhibition | Solanum lycopersicum | |
EDTA | 0.001 mM: 30% inhibition, 0.01 mM: complete inactivation | Solanum lycopersicum | |
iodoacetate | 0.001-0.01 mM: 50-70% inhibition | Solanum lycopersicum | |
Mg2+ | 0.1-10 mM: 20-50% inhibition | Solanum lycopersicum | |
Mn2+ | 0.1-10 mM: 20-80% inhibition | Solanum lycopersicum | |
PCMB | 0.001-0.01 mM: 50-70% inhibition | Solanum lycopersicum | |
UDP | 1 mM, 70% inhibition after 60 min | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
p-coumaric acid | - |
Solanum lycopersicum | |
0.0014 | - |
ferulic acid | - |
Solanum lycopersicum | |
0.0015 | - |
caffeic acid | - |
Solanum lycopersicum | |
0.0025 | - |
Sinapic acid | - |
Solanum lycopersicum | |
0.01 | - |
UDPglucose | reaction with ferulic acid | Solanum lycopersicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
MnCl2 | 0.006-0.01 mM: stimulation of about 10% | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
var. cerasiforme | - |
Purification (Comment) | Organism |
---|---|
partial | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00112 | - |
reaction with ferulic acid | Solanum lycopersicum |
Storage Stability | Organism |
---|---|
-20°C, purified enzyme, complete loss of activity after 12-24 h | Solanum lycopersicum |
0-4°C, purified enzyme, 40% loss of activity after 24 h, 70% loss of activity after 48 h | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + sinapic acid | - |
Solanum lycopersicum | UDP + 4-O-beta-D-glucosyl-sinapic acid + 1-O-sinapoyl-beta-D-glucose | a mixture of the glucoside and the glucose ester is formed | ? | |
UDPglucose + aesculin | - |
Solanum lycopersicum | UDP + 4-O-beta-D-glucosyl-aesculin | - |
? | |
UDPglucose + caffeic acid | - |
Solanum lycopersicum | UDP + 4-O-glucosyl-caffeic acid | no glucose ester is formed | ? | |
UDPglucose + daphnetin | - |
Solanum lycopersicum | UDP + 4-O-beta-D-glucosyl-daphnetin | - |
? | |
UDPglucose + ferulic acid | - |
Solanum lycopersicum | UDP + feruloyl-beta-D-glucose + 4-O-glucosyl-ferulic acid | a mixture of the glucoside and the glucose ester is formed | ? | |
UDPglucose + kaempferol | - |
Solanum lycopersicum | UDP + 4-O-beta-D-glucosyl-kaempferol | - |
? | |
UDPglucose + p-coumaric acid | - |
Solanum lycopersicum | UDP + p-coumaroyl-beta-D-glucose + 4-O-glucosyl-p-coumaric acid | a mixture of the glucoside and the glucose ester is formed | ? | |
UDPglucose + quercetin | - |
Solanum lycopersicum | UDP + 4-O-beta-D-glucosyl-quercetin | - |
? | |
UDPglucose + scopoletin | - |
Solanum lycopersicum | UDP + 4-O-beta-D-glucosyl-scopoletin | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
formation of glucose ester of p-coumaric acid | Solanum lycopersicum |
8 | - |
formation of glucoside of p-coumaric acid | Solanum lycopersicum |