Cloned (Comment) | Organism |
---|---|
a soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
E100Q | mutant of the soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT. glycosyltransferase activity of the mutant is reduced 500fold compared to the wild type | Staphylococcus aureus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | the enzyme is almost inactive in presence of EDTA | Staphylococcus aureus | |
Moenomycin | 75 nM, 50% inhibition | Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mn2+ | Staphylococcus aureus | |
0.0028 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 6.0, 10 mM Mn2+ | Staphylococcus aureus | |
0.0076 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 50 mM Mg2+ | Staphylococcus aureus | |
0.008 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mg2+ | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, stimulation | Staphylococcus aureus | |
Mg2+ | 50 mM, required for optimal activity | Staphylococcus aureus | |
Mn2+ | 10 mM required for optimal activity | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
soluble form of Staphylococcus aureus MGT devoid of its membrane anchor, called SauH6-MGT (D68-R268), is expressed in the cytoplasm of Escherichia coli C41 (DE3) strain | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc + undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | - |
Staphylococcus aureus | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 6.0, 10 mM Mn2+ | Staphylococcus aureus | |
0.0076 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 50 mM Mg2+ | Staphylococcus aureus | |
0.008 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mg2+ | Staphylococcus aureus | |
0.013 | - |
undecaprenyl-diphosphoryl-MurNAc-(L-Ala-gamma-D-Glu-meso-(diaminopimelic acid)-D-Ala-D-Ala)-GlcNAc | pH 7.5, 10 mM Mn2+ | Staphylococcus aureus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Staphylococcus aureus |